Login Register Cart 0
Generic placeholder image

Recent Advances in Anti-Infective Drug Discovery

Editor-in-Chief

ISSN (Print): 2772-4344
ISSN (Online): 2772-4352

Back
Journal Home About Journal Editorial Board Current Issue Volumes/Issues
Subscribe
Research Article

Frequency of blaIMP and blaSPM Metallo-β-Lactamase Genes among Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolates in Sari, North of Iran

Author(s): Zahra Norouzi Bazgir, Mohammad Ahanjan*, Hamid Reza Goli*, Mehrdad Gholami, Roya Ghasemian and Mohammad Bagher Hashemi-Soteh

Volume 16, Issue 2, 2021

Published on: 29 July, 2021

Page: [148 - 156] Pages: 9

DOI: 10.2174/2772434416666210607141520

Price: $65

Abstract

Introduction: Metallo-β-lactamases (MBLs) play a major role in the resistance of Pseudomonas aeruginosa to carbapenems. We investigated the antibiotic susceptibility patterns and frequency of MBLs genes (bla and blaSPM) in carbapenem-resistant P. aeruginosa clinical isolates in Sari, Iran.

Materials and Methods: The isolates were identified using standard microbiological tests, and their antibiotic susceptibility pattern was determined by the disk agar diffusion method according CLSI criteria. Phenotypic identification of MBL-producing strains assessed by the combined disk test (CDT). Then, polymerase chain reaction (PCR) was used to detect the presence of bla and blaSPM genes.

Results: The highest and lowest levels of antibiotic resistance were observed against gentamicin (40%) and piperacillin-tazobactam (13%), respectively. Besides, 40 isolates (40%) had the multi-drug resistant (MDR) phenotype, while 5 (12.5%) MDR isolates were resistant to all antibiotics tested. The results of the CDT showed that among 43 carbapenem non-susceptible clinical isolates of P. aeruginosa, 33 (76.74%) isolates were MBL-producing strains. Also, the frequency of the blaIMP gene among 43 carbapenem non susceptible isolates was determined to be 6.97%, while none of these isolates carried the blaSPM gene.

Conclusion: Due to the high prevalence of carbapenem-resistant and MDR P. aeruginosa in this study, routine antibiotic susceptibility testing and phenotypic identification of carbapenemase production by this bacterium are necessary for the proper selection of antibiotics.

Keywords: Pseudomonas aeruginosa, Carbapenem, Metallo-beta-lactamase, IMP, SPM, blaIMP and blaSPM.

« Previous Next »
Graphical Abstract

[1]
Diggle SP, Whiteley M. Microbe profile: Pseudomonas aeruginosa: Opportunistic pathogen and lab rat. Microbiology 2020; 166(1): 30-3.
[http://dx.doi.org/10.1099/mic.0.000860] [PMID: 31597590]
[2]
Chatterjee M, Anju CP, Biswas L, Anil KV, Gopi MC, Biswas R. Antibiotic resistance in Pseudomonas aeruginosa and alternative therapeutic options. Int J Med Microbiol 2016; 306(1): 48-58.
[http://dx.doi.org/10.1016/j.ijmm.2015.11.004] [PMID: 26687205]
[3]
Mohammadzadeh A, Mardaneh J, Ahmadi R, Adabi J. Evaluation of the virulence features and antibiotic resistance patterns of pathogenic Pseudomonas aeruginosa strains isolated from hospitalized patients in Gonabad, Iran. Arch Pediatr Infect Dis 2017; 5(3): e41267.
[http://dx.doi.org/10.5812/pedinfect.41267]
[4]
Goli HR, Nahaei MR, Ahangarzadeh RM, Hasani A, Samadi KH, Aghazadeh M. Emergence of colistin resistant Pseudomonas aeruginosa at Tabriz hospitals, Iran. Iran J Microbiol 2016; 8(1): 62-9.
[PMID: 27092226]
[5]
Mirsalehian A, Kalantar-Neyestanaki D, Taherikalani M, Jabalameli F, Emaneini M. Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran. J Epidemiol Glob Health 2017; 7(3): 155-9.
[http://dx.doi.org/10.1016/j.jegh.2017.04.002] [PMID: 28756823]
[6]
Amini A, Namvar AE. Antimicrobial resistance pattern and presence of beta-lactamase genes in Pseudomonas aeruginosa strains isolated from hospitalized patients, Babol-Iran. J Med Bacteriol 2019; 8(1, 2): 45-50.
[7]
Sedighi M, Hasanzadeh A, Safiri S, Syedi N, Mostafaei S, Faghri J. Detection of blaspm-1 metallo-β-lactamase gene in imipenem-resistant Pseudomonas aeruginosa strains isolated from hospitalized patients in Isfahan Hospitals. J Arch Mil Med 2015; 3(2): 26977.
[http://dx.doi.org/10.5812/jamm.3(2)2015.26977]
[8]
Yousefi S, Farajnia S, Nahaei MR, et al. Detection of metallo-β-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran. Diagn Microbiol Infect Dis 2010; 68(3): 322-5.
[http://dx.doi.org/10.1016/j.diagmicrobio.2010.06.018] [PMID: 20846807]
[9]
Seok Y, Bae IK, Jeong SH, Kim SH, Lee H, Lee K. Dissemination of IMP-6 metallo-β-lactamase-producing Pseudomonas aeruginosa sequence type 235 in Korea. J Antimicrob Chemother 2011; 66(12): 2791-6.
[http://dx.doi.org/10.1093/jac/dkr381] [PMID: 21933788]
[10]
Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-β-lactamases: The quiet before the storm? Clin Microbiol Rev 2005; 18(2): 306-25.
[http://dx.doi.org/10.1128/CMR.18.2.306-325.2005] [PMID: 15831827]
[11]
Clinical and laboratory standards institute (CLSI): Performance standards for antimicrobial susceptibility testing. Twenty-seventh informational supplement, M100-S28 2018. Available from: http://file.qums.ac.ir/repository/mmrc/CLSI-2018-M100-S28.pdf. (Accessed date: 1 July, 2021).
[12]
Kazeminezhad B, Bostanmanesh Rad A, Gharib A, Zahedifard S. blaVIM and blaIMP genes detection in isolates of carbapenem resistant P. aeruginosa of hospitalized patients in two hospitals in Iran. Iran J Pathol 2017; 12(4): 392-6.
[http://dx.doi.org/10.30699/ijp.2017.28323] [PMID: 29563936]
[13]
Radan M, Moniri R, Khorshidi A, et al. Emerging carbapenem-resistant Pseudomonas aeruginosa isolates carrying blaIMP among burn patients in Isfahan, Iran. Arch Trauma Res 2016; 5(3): e33664.
[http://dx.doi.org/10.5812/atr.33664] [PMID: 27800466]
[14]
Cho HH, Kwon GC, Kim S, Koo SH. Distribution of pseudomonas-derived Cephalosporinase and Metallo-β-lactamases in Carbapenem-resistant Pseudomonas aeruginosa isolates from Korea. J Microbiol Biotechnol 2015; 25(7): 1154-62.
[http://dx.doi.org/10.4014/jmb.1503.03065] [PMID: 25907063]
[15]
Phillips PL, Schultz GS. Molecular mechanisms of biofilm infection: biofilm virulence factors. Adv Wound Care 2012; 1(3): 109-14.
[http://dx.doi.org/10.1089/wound.2011.0301] [PMID: 24527289]
[16]
Safari M, Alikhani MY, Arabestani MR, Kamali Kakhki R, Jafari R. Prevalence of Metallo-β-lactamases encoding genes among Pseudomonas aeruginosa strains isolated from the bedridden patients in the intensive care units. Avicenna J Clin Microbiol Infect 2014; 1(1): e19216.
[http://dx.doi.org/10.17795/ajcmi-19216]
[17]
Alikhani MY, Parsavash S, Arabestani MR, Hosseini SM. Prevalence of antibiotic resistance and class 1 integrons in clinical and environmental isolates of Pseudomonas aeruginosa. Avicenna J Clin Microbiol Infect 2017; 4(4): 12086.
[18]
Nikokar I, Tishayar A, Flakiyan Z, et al. Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa, isolated from burn patients in Guilan, Iran. Iran J Microbiol 2013; 5(1): 36-41.
[PMID: 23466812]
[19]
Ismail SJ, Mahmoud SS. First detection of New Delhi metallo-β-lactamases variants (NDM-1, NDM-2) among Pseudomonas aeruginosa isolated from Iraqi hospitals. Iran J Microbiol 2018; 10(2): 98-103.
[PMID: 29997749]
[20]
Arunagiri K, Sekar B, Sangeetha G, John J. Detection and characterization of metallo-β-lactamases in Pseudomonas aeruginosa by phenotypic and molecular methods from clinical samples in a tertiary care hospital. West Indian Med J 2012; 61(8): 778-83.
[PMID: 23757897]
[21]
Shakibaie MR, Shahcheraghi F, Hashemi A, Adeli NS. Detection of TEM, SHV and PER type extended-spectrum β-Lactamase genes among clinical strains of Pseudomonas aeruginosa isolated from burnt patients at Shafa-Hospital, Kerman, Iran. Iran J Basic Med Sci 2008; 11(2): 104-11.
[22]
Attal R, Basak S, Mallick S, Bose S. Metallobetalactamase producing Pseudomonas aeruginosa: An emerging threat to clinicians. J Clin Diagn Res 2010; 4: 2691-6.
[23]
Polotto M, Casella T, de Lucca Oliveira MG, et al. Detection of P. aeruginosa harboring bla CTX-M-2, bla GES-1 and bla GES-5, bla IMP-1 and bla SPM-1 causing infections in Brazilian tertiary-care hospital. BMC Infect Dis 2012; 12(1): 176.
[http://dx.doi.org/10.1186/1471-2334-12-176] [PMID: 22863113]
[24]
Pitout JD, Chow BL, Gregson DB, Laupland KB, Elsayed S, Church DL. Molecular epidemiology of metallo-β-lactamase-producing Pseudomonas aeruginosa in the Calgary Health Region: Emergence of VIM-2-producing isolates. J Clin Microbiol 2007; 45(2): 294-8.
[http://dx.doi.org/10.1128/JCM.01694-06] [PMID: 17122002]
[25]
Zafer MM, Al-Agamy MH, El-Mahallawy HA, Amin MA, Ashour MSE-D. Antimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa strains isolated from cancer patients. BioMed Res Int 2014; 2014: 101635.
[26]
Xu Y, Niu H, Hu T, et al. High expression of metallo-β-lactamase contributed to the resistance to carbapenem in clinical isolates of Pseudomonas aeruginosa from Baotou, China. Infect Drug Resist 2020; 13: 35-43.
[http://dx.doi.org/10.2147/IDR.S233987] [PMID: 32021318]
[27]
Azimi A, Peymani A, Pour PK. Phenotypic and molecular detection of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates from patients with burns in Tehran, Iran. Rev Soc Bras Med Trop 2018; 51(5): 610-5.
[http://dx.doi.org/10.1590/0037-8682-0174-2017] [PMID: 30304266]

Rights & Permissions Print Cite
Article Metrics
6
1
© 2024 Bentham Science Publishers | Privacy Policy