Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

Title:Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

Volume: 22 Issue: 39

Author(s): Marcela Cristina de Moraes, Carmen Lucia Cardoso, Cláudia Seidl, Ruin Moaddel and Quezia Bezerra Cass

Affiliation:

Keywords: Anti-cancer active compounds, screening method, affinity-based chromatography assays.

Abstract: Affinity-based chromatography assays encompass the use of solid supports containing immobilized biological targets to monitor binding events in the isolation, identification and/or characterization of bioactive compounds. This powerful bioanalytical technique allows the screening of potential binders through fast analyses that can be directly performed using isolated substances or complex matrices. An overview of the recent researches in frontal and zonal affinity-based chromatography screening assays, which has been used as a tool in the identification and characterization of new anti-cancer agents, is discussed. In addition, a critical evaluation of the recently emerged ligands fishing assays in complex mixtures is also discussed.

Cite this article as:

Moraes de Marcela Cristina, Cardoso Lucia Carmen, Seidl Cláudia, Moaddel Ruin and Cass Bezerra Quezia, Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays, Current Pharmaceutical Design 2016; 22 (39) . https://dx.doi.org/10.2174/1381612822666160614080506