Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Peptidoglycan Binding Region of the Ser/Thr Kinase PrkC from Staphylococcus aureus

Title: Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Peptidoglycan Binding Region of the Ser/Thr Kinase PrkC from Staphylococcus aureus

Volume: 17 Issue: 10

Author(s): Alessia Ruggiero, Flavia Squeglia, Viviana Izzo, Alba Silipo, Luigi Vitagliano, Antonio Molinaro and Rita Berisio

Affiliation:

Keywords: Crystal, cell wall, X-ray, latency

Abstract: PrkC is an important Ser/Thr membrane kinase of Staphylococcus aureus able to bind peptidoglycans through extra-cellular domains, denominated as PASTA. Upon peptidoglycan binding, PrkC is activated and stimulates bacterial growth and revival from latency. The entire extra-cellular region of PrkC (residues 378-664), containing three predicted PASTA domains and an extra-domain of unknown function, has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and refinement is in progress.

Cite this article as:

Ruggiero Alessia, Squeglia Flavia, Izzo Viviana, Silipo Alba, Vitagliano Luigi, Molinaro Antonio and Berisio Rita, Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Peptidoglycan Binding Region of the Ser/Thr Kinase PrkC from Staphylococcus aureus, Protein & Peptide Letters 2010; 17 (10) . https://dx.doi.org/10.2174/092986610792231401