Preparation of a Tat-Related Transporter Peptide for Carrying the Adenovirus Vector into Cells

Title: Preparation of a Tat-Related Transporter Peptide for Carrying the Adenovirus Vector into Cells

Volume: 15 Issue: 2

Author(s): Koichi Kawasaki, Shinya Kida, Yusuke Eto, Mitsuko Maeda, Yasuo Yoshioka, Shinsaku Nakagawa, Keiko Hojo, Yuko Tsuda and Tadanori Mayumi

Affiliation:

Keywords: Adenovirus, cell-penetrating peptide, Tat peptide, synthetic peptide

Abstract: We synthesized a Tat-related peptide acetyl-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-Gly- Cys amide, Ac-Tat48-60-Gly-Cys-NH2, having high intracellular permeability, and conjugated this peptide to adenovirus vector to enhance gene transfer efficiency of adenovirus vector into cells. The peptide was prepared by the solid-phase peptide synthesis method and a bifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide ester was used to conjugate the peptide to adenovirus vector containing luciferase gene. The novel conjugate of adenovirus vector and Ac-Tat48-60-Gly-Cys-NH2 peptide exhibited excellent gene transfer efficacy in B16BL6 cells.

Cite this article as:

Kawasaki Koichi, Kida Shinya, Eto Yusuke, Maeda Mitsuko, Yoshioka Yasuo, Nakagawa Shinsaku, Hojo Keiko, Tsuda Yuko and Mayumi Tadanori, Preparation of a Tat-Related Transporter Peptide for Carrying the Adenovirus Vector into Cells, Protein & Peptide Letters 2008; 15 (2) . https://dx.doi.org/10.2174/092986608783489526