Abstract
Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out posttranslational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.
Keywords: Cell-free protein synthesis, 5'-UTR, Freeze-thawing, Insect cell, MALDI-TOF MS, Post-translational modification, Rabbit reticulocyte
Current Pharmaceutical Biotechnology
Title: Development of an Insect Cell-Free System
Volume: 11 Issue: 3
Author(s): Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, Eiji Ando, Toshihiko Utsumi, Osamu Nishimura and Susumu Tsunasawa
Affiliation:
Keywords: Cell-free protein synthesis, 5'-UTR, Freeze-thawing, Insect cell, MALDI-TOF MS, Post-translational modification, Rabbit reticulocyte
Abstract: Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out posttranslational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.
Export Options
About this article
Cite this article as:
Ezure Toru, Suzuki Takashi, Shikata Masamitsu, Ito Masaaki, Ando Eiji, Utsumi Toshihiko, Nishimura Osamu and Tsunasawa Susumu, Development of an Insect Cell-Free System, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791111997
DOI https://dx.doi.org/10.2174/138920110791111997 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Celecoxib A Selective COX-2 Inhibitor Mitigates Fibrosis but not Pneumonitis Following Lung Irradiation: A Histopathological Study
Current Drug Therapy Subject Index To Volume 5
CNS & Neurological Disorders - Drug Targets Activity Based Chemical Proteomics: Profiling Proteases as Drug Targets
Current Drug Discovery Technologies Regulation of V-ATPase Expression in Mammalian Cells
Current Protein & Peptide Science Light Directed Gene Transfer by Photochemical Internalisation
Current Gene Therapy Rational Design and Synthesis of Benzamides as Non-ulcerogenic Anti-inflammatory Agents
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry 4-Hydroxynonenal in the Pathogenesis and Progression of Human Diseases
Current Medicinal Chemistry Patent Selections
Recent Patents on Anti-Infective Drug Discovery The Use of Hematopoietic Growth Factors in the Treatment of Acute Leukemia
Current Pharmaceutical Design Ovarian Cancer Biomarkers: Headway Towards Early Diagnosis
Current Chemical Biology Design, Synthesis and Biological Evaluation of New 1,3-diphenyl-3- (phenylamino)propan-1-ones as Selective Cyclooxygenase (COX-2) Inhibitors
Medicinal Chemistry The Role of Heme Oxygenase-1 in T Cell-Mediated Immunity: The All Encompassing Enzyme
Current Pharmaceutical Design Enteral and Parenteral Nutrition Therapy for Crohns Disease
Current Pharmaceutical Design Strategies for Leukotriene Modulation in Dermatology: Even More Visionary Perspectives? An Update
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Advances in Drug Therapy for Systemic Lupus Erythematosus
Current Medicinal Chemistry Understanding and Modulating the Toll Like Receptors (TLRs) and NOD Like Receptors (NLRs) Cross Talk in Type 2 Diabetes
Current Diabetes Reviews Cancer Vaccines for Hormone/Growth Factor Immune Deprivation:A Feasible Approach for Cancer Treatment
Current Cancer Drug Targets Apoptotic Effects of N-(2-Hydroxyphenyl)-2-Propylpentanamide on U87-MG and U-2 OS Cells and Antiangiogenic Properties
Anti-Cancer Agents in Medicinal Chemistry The Effects of Erythropoietin on the Respiratory Function: Measurements of Respiratory Mechanics in the Rat
Current Respiratory Medicine Reviews Assessment of Circulating Biomarkers in Relation to Various Stages of Diabetic Retinopathy in Type 2 Diabetic Patients-A Cross Sectional Study
Current Diabetes Reviews