Step-Wise Refolding of Recombinant Proteins

Title: Step-Wise Refolding of Recombinant Proteins

Volume: 11 Issue: 3

Author(s): Kouhei Tsumoto, Tsutomu Arakawa and Linda Chen

Affiliation:

Keywords: Step-wise refolding, arginine, aggregation, antibody fragment, dialysis

Abstract: Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.

Cite this article as:

Tsumoto Kouhei, Arakawa Tsutomu and Chen Linda, Step-Wise Refolding of Recombinant Proteins, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791111979