Evaluation of Anti-Tumor Potential of Lactobacillus acidophilus ATCC4356 Culture Supernatants in MCF-7 Breast Cancer

Title:Evaluation of Anti-Tumor Potential of Lactobacillus acidophilus ATCC4356 Culture Supernatants in MCF-7 Breast Cancer

Volume: 21 Issue: 14

Author(s): Ramazan Behzadi, Ahmad Hormati, Karim Eivaziatashbeik, Sajjad Ahmadpour*, Fatemeh Khodadust, Fatemeh Zaboli, Esmail Fattahi, Rana Jahanban-­Esfahlan and Khaled Seidi

Affiliation:

• Gastroenterology and Hepatology Diseases Research Center, Qom University of Medical Sciences, Qom,Iran

Keywords: Breast cancer, probiotics, MTT assay, histopathology, ^99mTc-MIBI, biodistribution.

Abstract:

Background: The anti-cancer activity of some lactic acid bacterial strains is well documented in several kinds of literatures. Lactobacillus strains have received considerable attention as a beneficial microbiota. The aim of this study is to evaluate the effects of anti-tumor activities of L. acidophilus ATCC4356 culture supernatants on the MCF-7 human breast cancer cells.

Materials and Methods: The anti-cancer effects of 24h and 48h culture supernatants at various concentrations (1.25, 2.5, 5, 10 and 20 μg/ml) were determined by various in vitro and in vivo assays including MTT, tumor volume measurement as well as ^99mTc-MIBI biodistribution in MCF-7 tumor bearing nude mice and histopathology test. For evaluation of the related mechanism of action, quantitative PCR was conducted.

Results: The 48h culture supernatants at 10 and 20 μg/ml exhibited significant in vitro inhibition of MCF-7 cell proliferation. However, this inhibition was not observed for HUVEC human endothelial normal cells. Q-PCR indicated that treatment by the supernatant led to a significant downregulation of VEGFR (~ 0.009 fold) and Bcl- 2 (~ 0.5 fold) and upregulation of p53 (~ 1.3 fold). In vivo study using MCF-7 xenograft mouse models demonstrated a reduction in tumor weight and volume by both 24h and 48h supernatants (2 mg/kg) after 15 days. According to the ^99mTc-MIBI biodistribution result, treatment of MCF-7 bearing nude mice with both 24h and 48h supernatant (2mg/kg) led to a significant decrease in tumor uptake compared with the control group.

Conclusion: These results suggest that the culture supernatants of L. acidophilus ATCC4356 at suitable concentrations can be considered as a good alternative nutraceutical with promising therapeutic indexes for breast cancer.

Cite this article as:

Behzadi Ramazan , Hormati Ahmad , Eivaziatashbeik Karim , Ahmadpour Sajjad *, Khodadust Fatemeh, Zaboli Fatemeh , Fattahi Esmail , Jahanban-­Esfahlan Rana and Seidi Khaled , Evaluation of Anti-Tumor Potential of Lactobacillus acidophilus ATCC4356 Culture Supernatants in MCF-7 Breast Cancer, Anti-Cancer Agents in Medicinal Chemistry 2021; 21 (14) . https://dx.doi.org/10.2174/1871520621666201207085239