Abstract
Background: Dillenia indica (Family: Dilleniaceae) is an antioxidant-rich edible fruitbearing medicinal plant. The fruit of this plant (known as elephant apple) has many uses in traditional medicine.
Objective: By considering its antioxidant content and ameliorating effects, this study aimed to evaluate the anti-adipogenic effects of D. indica fruit extract (DIFE) on high-fat diet (HFD) fed obese rats.
Methods: Male Wistar rats were fed with a standard diet (SD), or high-fat diet (HFD), or HFD with 100 mg/kg or 200 mg/kg or 400 mg/kg DIFE for 8 weeks. The fruit extract was given orally by feeding gavage. The body weight, liver weight, visceral fat weight, plasma lipids, and oxidative stress-related parameters were measured. The mRNA level of different adipogenesis related transcription factors, lipogenic and lipolytic enzymes was also evaluated.
Results: Consumption of DIFE daily (400 mg/kg) for 8 weeks resulted in a significant reduction of high-fat diet-induced body weight, liver weight, visceral fat weight, total cholesterol, and LDL-cholesterol level. High-fat diet-mediated elevation of oxidative stress markers was also lowered, with a parallel augmentation of the activities of antioxidant enzymes, due to 400 mg/kg DIFE feeding. DIFE also down-regulated the mRNA level of important pro-adipogenic factors like PPARγ, LXRα, and SREBP1c which consequently downregulated the transcript levels of lipogenic enzymes: ACC, FAS, HMGCR, and DGAT. The transcript level of lipolytic enzyme, HSL was also down-regulated in 400 mg/kg DIFE-fed rats.
Conclusion: These findings indicate that the antioxidant-rich ethanolic extract of D. indica fruit can down-regulate the gene expression of pro-adipogenic transcription factors and lipid metabolizing enzymes and thus can suppress diet-induced obesity in Wistar rat.
Keywords: Dillenia indica, adipogenesis, dyslipidemia, LDL-cholesterol, oxidative stress, gene expression, transcription factors.
Graphical Abstract
[http://dx.doi.org/10.1007/s00394-019-01916-7] [PMID: 30796508]
[http://dx.doi.org/10.1111/j.1745-4514.2009.00269.x]
[http://dx.doi.org/10.1111/1750-3841.13005] [PMID: 26416622]
[http://dx.doi.org/10.1155/2019/4632491] [PMID: 31871947]
[http://dx.doi.org/10.1016/j.jep.2009.02.037] [PMID: 19429357]
[http://dx.doi.org/10.1016/j.phymed.2009.07.010] [PMID: 19679456]
[http://dx.doi.org/10.1016/S1995-7645(11)60101-6] [PMID: 21771674]
[http://dx.doi.org/10.1016/j.nut.2016.07.018] [PMID: 27746034]
[http://dx.doi.org/10.1016/j.jff.2016.02.016]
[http://dx.doi.org/10.1016/j.foodchem.2004.09.002]
[http://dx.doi.org/10.1080/10942911003592779]
[http://dx.doi.org/10.1139/apnm-2017-0461] [PMID: 29091744]
[http://dx.doi.org/10.2174/1574888X10666150528144905] [PMID: 26018229]
[http://dx.doi.org/10.1530/JOE-16-0142] [PMID: 27288055]
[http://dx.doi.org/10.1186/s12906-015-0598-3] [PMID: 25887331]
[http://dx.doi.org/10.1371/journal.pone.0070058] [PMID: 23894592]
[http://dx.doi.org/10.1155/2013/492807] [PMID: 23365609]
[http://dx.doi.org/10.1111/j.1463-1326.2010.01275.x] [PMID: 21029304]
[http://dx.doi.org/10.1155/2014/253875] [PMID: 24782905]
[http://dx.doi.org/10.1111/jfbc.12556]
[http://dx.doi.org/10.1016/j.apjtb.2016.12.007]
[http://dx.doi.org/10.1248/bpb.24.1202] [PMID: 11642334]
[http://dx.doi.org/10.1186/s12906-017-1712-5] [PMID: 28376770]
[http://dx.doi.org/10.1155/2013/210297] [PMID: 23818922]
[http://dx.doi.org/10.1007/s11418-010-0461-2] [PMID: 20814753]
[http://dx.doi.org/10.1248/bpb.31.1415] [PMID: 18591785]
[http://dx.doi.org/10.1155/2016/9412767] [PMID: 27123360]
[http://dx.doi.org/10.1111/bph.12832] [PMID: 24990201]
[http://dx.doi.org/10.1016/j.biocel.2004.01.028] [PMID: 15203107]
[http://dx.doi.org/10.1139/apnm-2016-0454] [PMID: 28177723]
[http://dx.doi.org/10.1186/s12858-018-0097-5] [PMID: 30075706]
[http://dx.doi.org/10.1001/jama.2013.280532] [PMID: 24240933]
[http://dx.doi.org/10.1177/1179546818759286] [PMID: 29497341]
[http://dx.doi.org/10.1016/j.foodchem.2018.06.020] [PMID: 30381205]
[http://dx.doi.org/10.3389/fimmu.2018.02649] [PMID: 30483273]
[http://dx.doi.org/10.3389/fnut.2018.00017] [PMID: 29616223]
[http://dx.doi.org/10.1007/s00394-018-1643-z] [PMID: 29476238]
[http://dx.doi.org/10.1097/00041433-200108000-00007] [PMID: 11507326]
[http://dx.doi.org/10.1111/jfbc.12939] [PMID: 31368568]
[http://dx.doi.org/10.4239/wjd.v6.i3.456] [PMID: 25897356]
[http://dx.doi.org/10.1042/BSR20181665] [PMID: 30842338]
[http://dx.doi.org/10.1007/s00394-017-1599-4] [PMID: 29274033]
[http://dx.doi.org/10.3109/13880209.2013.872672] [PMID: 24766363]
[http://dx.doi.org/10.1017/S000711450774686X] [PMID: 17475086]
[http://dx.doi.org/10.1016/j.jtcme.2017.06.004] [PMID: 29322013]
[http://dx.doi.org/10.1186/s12906-017-1640-4] [PMID: 28228098]
[http://dx.doi.org/10.1371/journal.pone.0096969] [PMID: 24918580]
[http://dx.doi.org/10.1155/2016/4353604] [PMID: 28058058]
[http://dx.doi.org/10.1128/MCB.01344-13] [PMID: 24379442]
[http://dx.doi.org/10.1007/s00394-018-1663-8] [PMID: 29541908]
[http://dx.doi.org/10.1111/j.1600-065X.2012.01153.x] [PMID: 22889216]
[http://dx.doi.org/10.1038/s41467-018-07590-3] [PMID: 30510211]
[http://dx.doi.org/10.3390/molecules24061045] [PMID: 30884812]
[http://dx.doi.org/10.1373/clinchem.2008.115352] [PMID: 19181734]
[http://dx.doi.org/10.1194/jlr.R800018-JLR200] [PMID: 18757836]
[PMID: 15498253]
[PMID: 28232747]
[http://dx.doi.org/10.1002/mnfr.201700139] [PMID: 28556482]