Naringenin and Ellagic Acid Reduce Tetrazolium Salt in the Absence of Cells

Title:Naringenin and Ellagic Acid Reduce Tetrazolium Salt in the Absence of Cells

Volume: 11 Issue: 4

Author(s): Vaibhav Tiwary, Priyanka Sarkar and Kavitha Thirumurugan*

Affiliation:

• Structural Biology Lab, Center for Bio Medical Research, School of Bio Sciences Technology, Vellore Institue of Technology, Tamil Nadu,India

Keywords: Naringenin, ellagic acid, MTT, absorbance, absence of cells, phytocompounds.

Abstract:

Background: Plant derived compounds, naringenin and ellagic acid might interfere in MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay in the form of reducing the tetrazolium salt in the absence of cells.

Objective: Effect of naringenin and ellagic acid in MTT assay.

Methods: MTT assay has been performed in different conditions like, varying the concentration of phytocompounds, incubation time, and changing the media.

Results: Ellagic acid at the concentration of 80 μg/ml showed maximum absorbance of 0.579 in the presence of RPMI media (Roswell Park Memorial Institute Medium) that has 10% newborn calf serum. Naringenin showed higher absorbance of 2.74 at 80 μg/ml in RPMI media that has 10% newborn calf serum. Naringenin and Ellagic acid had better ability to reduce the MTT in the presence of RPMI media than anhydrous ethanol.

Conclusion: Naringenin reduced the MTT better than ellagic acid in RPMI-1640 medium. These results show the need to be cautious when choosing MTT assay to measure the metabolic activity of cells treated with phytocompounds.

Cite this article as:

Tiwary Vaibhav , Sarkar Priyanka and Thirumurugan Kavitha *, Naringenin and Ellagic Acid Reduce Tetrazolium Salt in the Absence of Cells, The Natural Products Journal 2021; 11 (4) . https://dx.doi.org/10.2174/2210315510999200723162422