Login Register Cart 0
Generic placeholder image

Infectious Disorders - Drug Targets

Editor-in-Chief

ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

First Report on the Characteristics of Methicillin-Resistant Staphylococcus Capitis Isolates and an NRCS-A-clone Related Isolate Obtained from Iranian Children

Author(s): Parisa Asadollahi, Behrooz Farzan, Faranak Rezaei, Somayeh Delfani, Behnam Ashrafi and Setareh Soroush*

Volume 21, Issue 3, 2021

Published on: 10 May, 2020

Page: [459 - 463] Pages: 5

DOI: 10.2174/1871526520666200511011309

Price: $65

Abstract

Background: Methicillin-resistant staphylococcus capitis (MRSC) NRCS-A clone (Multi- resistant and vancomycin-non susceptible) has been recently described as an emerging cause of nosocomial bacteremia, especially in neonatal intensive-care units (NICUs).

Objective: The objective of this study was to evaluate the antibiotic and antiseptic resistance patterns, biofilm-producing ability and the prevalence of SCCmec and ACME types among MRSC isolates as well as to check the possible presence of NRCS-A clone at Tehran’s Children's Medical Center, Iran.

Methods: A total of 256 coagulase-negative Staphylococcal isolates were collected, of which 10 S. capitis isolates were obtained and tested for susceptibility against 13 antimicrobial and 3 antiseptic agents, as well as biofilm production. The presence of 15 distinct resistance genes, staphylococcal cassette chromosome mec (SCCmec), and arginine catabolic mobile elements (ACMEs) were tracked.

Results: Seven out of 10 S. capitis isolates were MRSC (MIC90 van=8μg/mL) and resistant to trimethoprim/sulfamethoxazole, produced biofilm, (3 as strong biofilm producers) and carried ACME types I and II. Despite the identification of mec and ccr complexes in some isolates, all the SCCmec cassettes were untypeable (UT).

Conclusion: According to the studied features, only one isolate belonged to the NRSC-A clone. The results indicate that MRSC with high antibiotic resistance and unknown SCCmec might become a serious problem in the future for the treatment of patients, particularly children.

Keywords: Staphylococcus capitis, NRCS-A clone, biofilm, SCCmec, ACME, CoNS.

Graphical Abstract

[1]
Kloos, W.E.; Schleifer, K.H. Simplified scheme for routine identification of human Staphylococcus species. J. Clin. Microbiol., 1975, 1(1), 82-88.
[http://dx.doi.org/10.1128/JCM.1.1.82-88.1975] [PMID: 170303]
[2]
Ben Said, M.; Hays, S.; Bonfils, M.; Jourdes, E.; Rasigade, J.P.; Laurent, F.; Picaud, J.C. Late-onset sepsis due to Staphylococcus capitis ‘neonatalis’ in low-birthweight infants: a new entity? J. Hosp. Infect., 2016, 94(1), 95-98.
[http://dx.doi.org/10.1016/j.jhin.2016.06.008] [PMID: 27424947]
[3]
Butin, M.; Martins-Simões, P.; Pichon, B.; Leyssene, D.; Bordes-Couecou, S.; Meugnier, H.; Rouard, C.; Lemaitre, N.; Schramm, F.; Kearns, A.; Spiliopoulou, I.; Hyyryläinen, H.L.; Dumitrescu, O.; Vandenesch, F.; Dupieux, C.; Laurent, F. Emergence and dissemination of a linezolid-resistant Staphylococcus capitis clone in Europe. J. Antimicrob. Chemother., 2017, 72(4), 1014-1020.
[PMID: 27999045]
[4]
Butin, M.; Rasigade, J.P.; Subtil, F.; Martins-Simões, P.; Pralong, C.; Freydière, A.M.; Vandenesch, F.; Tigaud, S.; Picaud, J.C.; Laurent, F. Vancomycin treatment is a risk factor for vancomycin-nonsusceptible Staphylococcus capitis sepsis in preterm neonates. Clin. Microbiol. Infect., 2017, 23(11), 839-844.
[http://dx.doi.org/10.1016/j.cmi.2017.03.022] [PMID: 28373147]
[5]
Butin, M.; Rasigade, J.P.; Martins-Simões, P.; Meugnier, H.; Lemriss, H.; Goering, R.V.; Kearns, A.; Deighton, M.A.; Denis, O.; Ibrahimi, A.; Claris, O.; Vandenesch, F.; Picaud, J.C.; Laurent, F. Wide geographical dissemination of the multiresistant Staphylococcus capitis NRCS-A clone in neonatal intensive-care units. Clin. Microbiol. Infect., 2016, 22(1), 46-52.
[http://dx.doi.org/10.1016/j.cmi.2015.09.008] [PMID: 26404028]
[6]
Winn, W.; Allen, S.; Janda, E.; Procop, G.; Koneman, W.G. Koneman’s color atlas and textbook of diagnostic microbiology, 6th ed; LWW. New York: Lippincott Williams and Wilkins; , 2005, 2, pp. 469-71.
[7]
Magiorakos, A.P.; Srinivasan, A.; Carey, R.B.; Carmeli, Y.; Falagas, M.E.; Giske, C.G.; Harbarth, S.; Hindler, J.F.; Kahlmeter, G.; Olsson-Liljequist, B.; Paterson, D.L.; Rice, L.B.; Stelling, J.; Struelens, M.J.; Vatopoulos, A.; Weber, J.T.; Monnet, D.L. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect., 2012, 18(3), 268-281.
[http://dx.doi.org/10.1111/j.1469-0691.2011.03570.x] [PMID: 21793988]
[8]
CLSI. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement., 2014, 34(M100-S24), 68e75.
[9]
Vali, L.; Davies, S.E.; Lai, L.L.; Dave, J.; Amyes, S.G. Frequency of biocide resistance genes, antibiotic resistance and the effect of chlorhexidine exposure on clinical methicillin-resistant Staphylococcus aureus isolates. J. Antimicrob. Chemother., 2008, 61(3), 524-532.
[http://dx.doi.org/10.1093/jac/dkm520] [PMID: 18227090]
[10]
Soroush, S.; Jabalameli, F.; Taherikalani, M.; Amirmozafari, N.; Fooladi, A.A.; Asadollahi, K.; Beigverdi, R.; Emaneini, M. Investigation of biofilm formation ability, antimicrobial resistance and the staphylococcal cassette chromosome mec patterns of methicillin resistant Staphylococcus epidermidis with different sequence types isolated from children. Microb. Pathog., 2016, 93, 126-130.
[http://dx.doi.org/10.1016/j.micpath.2016.01.018] [PMID: 26821355]
[11]
Caviedes, L.; Delgado, J.; Gilman, R.H. Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis. J. Clin. Microbiol., 2002, 40(5), 1873-1874.
[http://dx.doi.org/10.1128/JCM.40.5.1873-1874.2002] [PMID: 11980982]
[12]
Soroush, S.; Jabalameli, F.; Taherikalani, M.; Eslampour, M.A.; Beigverdi, R.; Emaneini, M. Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children. Rev. Soc. Bras. Med. Trop., 2017, 50(3), 329-333.
[http://dx.doi.org/10.1590/0037-8682-0384-2016] [PMID: 28700050]
[13]
Jabalameli, F.; Mirsalehian, A.; Khoramian, B.; Aligholi, M.; Khoramrooz, S.S.; Asadollahi, P.; Taherikalani, M.; Emaneini, M. Evaluation of biofilm production and characterization of genes encoding type III secretion system among Pseudomonas aeruginosa isolated from burn patients. Burns, 2012, 38(8), 1192-1197.
[http://dx.doi.org/10.1016/j.burns.2012.07.030] [PMID: 22995427]
[14]
Ito, T.; Kuwahara-Arai, K.; Katayama, Y.; Uehara, Y.; Han, X.; Kondo, Y.; Hiramatsu, K. Staphylococcal Cassette Chromosome mec (SCCmec) analysis of MRSA. Methods Mol. Biol., 2014, 1085, 131-148.
[http://dx.doi.org/10.1007/978-1-62703-664-1_8] [PMID: 24085694]
[15]
Diep, B.A.; Stone, G.G.; Basuino, L.; Graber, C.J.; Miller, A.; des Etages, S.A.; Jones, A.; Palazzolo-Ballance, A.M.; Perdreau-Remington, F.; Sensabaugh, G.F.; DeLeo, F.R.; Chambers, H.F. The arginine catabolic mobile element and staphylococcal chromosomal cassette mec linkage: convergence of virulence and resistance in the USA300 clone of methicillin-resistant Staphylococcus aureus. J. Infect. Dis., 2008, 197(11), 1523-1530.
[http://dx.doi.org/10.1086/587907] [PMID: 18700257]
[16]
Ehlersson, G.; Hellmark, B.; Svartström, O.; Stenmark, B.; Söderquist, B. Phenotypic characterisation of coagulase-negative staphylococci isolated from blood cultures in newborn infants, with a special focus on Staphylococcus capitis. Acta Paediatr., 2017, 106(10), 1576-1582.
[http://dx.doi.org/10.1111/apa.13950] [PMID: 28631328]
[17]
Rasigade, J.P.; Raulin, O.; Picaud, J.C.; Tellini, C.; Bes, M.; Grando, J.; Ben Saïd, M.; Claris, O.; Etienne, J.; Tigaud, S.; Laurent, F. Methicillin-resistant Staphylococcus capitis with reduced vancomycin susceptibility causes late-onset sepsis in intensive care neonates. PLoS One, 2012, 7(2), e31548.
[http://dx.doi.org/10.1371/journal.pone.0031548] [PMID: 22348102]
[18]
Tevell, S.; Hellmark, B.; Nilsdotter-Augustinsson, Å.; Söderquist, B. Staphylococcus capitis isolated from prosthetic joint infections. Eur. J. Clin. Microbiol. Infect. Dis., 2017, 36(1), 115-122.
[http://dx.doi.org/10.1007/s10096-016-2777-7] [PMID: 27680718]
[19]
Butin, M.; Martins-Simões, P.; Picaud, J.C.; Kearns, A.; Claris, O.; Vandenesch, F.; Laurent, F.; Rasigade, J.P. Adaptation to vancomycin pressure of multiresistant Staphylococcus capitis NRCS-A involved in neonatal sepsis. J. Antimicrob. Chemother., 2015, 70(11), 3027-3031.
[http://dx.doi.org/10.1093/jac/dkv217] [PMID: 26203181]
[20]
do Vale, B.C.M.; Nogueira, A.G.; Cidral, T.A.; Lopes, M.C.S.; de Melo, M.C.N.; Celeste, M. Decreased susceptibility to chlorhexidine and distribution of qacA/B genes among coagulase-negative Staphylococcus clinical samples. BMC Infect. Dis., 2019, 19(1), 199.
[http://dx.doi.org/10.1186/s12879-019-3823-8] [PMID: 30813914]
[21]
Natoli, S.; Fontana, C.; Favaro, M.; Bergamini, A.; Testore, G.P.; Minelli, S.; Bossa, M.C.; Casapulla, M.; Broglio, G.; Beltrame, A.; Cudillo, L.; Cerretti, R.; Leonardis, F. Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options. BMC Infect. Dis., 2009, 9(83), 83.
[http://dx.doi.org/10.1186/1471-2334-9-83] [PMID: 19497104]
[22]
Kratzer, C.; Rabitsch, W.; Hirschl, A.M.; Graninger, W.; Presterl, E. in vitro activity of daptomycin and tigecycline against coagulase-negative staphylococcus blood isolates from bone marrow transplant recipients. Eur. J. Haematol., 2007, 79(5), 405-409.
[http://dx.doi.org/10.1111/j.1600-0609.2007.00945.x] [PMID: 17714506]

Rights & Permissions Print Cite
Article Metrics
3
1
© 2024 Bentham Science Publishers | Privacy Policy