Characterization of Optimized Functional-Complementary Dual Insulinotorpic Peptide rolGG

Title:Characterization of Optimized Functional-Complementary Dual Insulinotorpic Peptide rolGG

Volume: 18 Issue: 10

Author(s): Yaofang Zhang, Li Du, Yanling Xu, Lin Tian, Peipei Tu*Minggang Li*

Affiliation:

• Biological Engineering Department, College of Life Science, Anhui Medical University, 230032 Hefei, Anhui,China

• State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, 300071 Tianjin,China

Keywords: GLP-1, GIP, diabetes, expression, purification, primers.

Abstract: Background: Glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP) play a similar but complementary role in the regulation of glucose levels in islet β-cells. This study was aimed to develop a fusion peptide, which combines 4 tandem repeated GLP-1 and 4 tandem repeated GIP (4rolGG), and to investigate its therapeutic effect on type 2 diabetes using a diabetic mice model.

Methods: A 4rolGG expression plasmid was constructed and expressed in BL21 (DE3). By inducting with IPTG, 4rolGG was expressed at a high level, which was confirmed by SDS-PAGE electrophoresis and Western Blotting. Subsequently, 4rolGG was purified by Ni-NTA affinity chromatography and the purity of 4rolGG was up to 90%.

Result and Conclusion: After oral administration of 4rolGG for 4 weeks, streptozotocin-induced diabetic mice showed a dramatic reduction in the levels of plasma glucose, GHbA1C, TC and TG, while the insulin levels were increased significantly.

Cite this article as:

Zhang Yaofang, Du Li , Xu Yanling, Tian Lin , Tu Peipei*, Li Minggang*, Characterization of Optimized Functional-Complementary Dual Insulinotorpic Peptide rolGG, Current Pharmaceutical Biotechnology 2017; 18 (10) . https://dx.doi.org/10.2174/1389201019666171204111908