Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

Title:Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

Volume: 3 Issue: 2

Author(s): Julia Baruque-Ramos, Luciana Juncioni de Arauz, Marcelo Fossa da Paz, Marcio Alberto Vicentin and Haroldo Hiss

Affiliation:

Keywords: Neisseria meningitidis, vaccine, polysaccharide, batch, fed-batch, kinetics.

Abstract: Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (P_f), as well as the overall yield factor (PS/biomass) (Y_P/X), 13 total cultivations distributed in 6 series (from A to F) were carried out in Frantz medium (40 L plus inoculum) in a 80L bioreactor at 35oC, 0.4 atm, 120 rpm, airflow rate of 5 L/min and K_La = 4.2 h^-1. The series (A-F) correspond to different experimental conditions as follows: A) without pH and dissolved O₂ controls; B) pH control at 6.5; C) pH control at 6.5 and glucose pulse at the 10th hour; D) dissolved O₂ control at 10% saturation value; E) pH control at 7.4; F) dissolved O₂ limitation (set rotation at 55 rpm). Concentrations of dry biomass, PS, cellular nitrogen, residual glucose, organic and inorganic nitrogen in the medium were measured. The best results were represented by series A (averages of P_f = 0.15 g/L and Y_P/X = 107 mg/g). The presented findings could be useful for a proper Frantz medium reformulation in order to obtain a greater amount of PS and improve the vaccine development in industrial scale-up production.

Cite this article as:

Baruque-Ramos Julia, Juncioni de Arauz Luciana, Fossa da Paz Marcelo, Alberto Vicentin Marcio and Hiss Haroldo, Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C, Current Biochemical Engineering (Discontinued) 2016; 3 (2) . https://dx.doi.org/10.2174/2212711902666150901200052