The Role of Tomatine Adjuvant in Antigen Delivery for Cross- Presentation

Title:The Role of Tomatine Adjuvant in Antigen Delivery for Cross- Presentation

Volume: 12 Issue: 3

Author(s): Paul Y.-J. Hsu, Ching-An Wu, Shan-Shan Shen and Ya-Wun Yang

Affiliation:

Keywords: Adjuvant, antigen delivery, tomatine, vaccine.

Abstract: The aim of this study was to investigate the use of tomatine adjuvant to deliver soluble antigen for crosspresentation by bone marrow-derived dendritic cells (BMDCs). BMDCs were incubated with tomatine adjuvantovalbumin (OVA) complex and analyzed for antigen uptake by flow cytometry. Adjuvant-induced cell death was examined in situ by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. To elucidate the effect of antigen internalization on tomatine adjuvant-mediated antigen presentation, BMDCs were treated with several endocytosis inhibitors, and antigen presentation was analyzed by B3Z activity assay. Our data indicated that tomatine adjuvant enhanced antigen internalization by antigen presenting cells (APCs) and induced significant cell death and leukocyte infiltration at the injection sites. In vitro tomatine adjuvant treatment of BMDCs activated Ova/K^b restricted B3Z T cell hybridomas, whereas this activation was impaired by pretreatment with brefeldin A, cytochalasin B, wortmannin, or ZnCl₂. Our results demonstrated the role of tomatine adjuvant in antigen delivery to antigen presenting cells (APCs) and suggested the involvement of phagocytosis and PI3K signaling during the delivery of soluble antigens in the context of MHC class I.

Cite this article as:

Hsu Y.-J. Paul, Wu Ching-An, Shen Shan-Shan and Yang Ya-Wun, The Role of Tomatine Adjuvant in Antigen Delivery for Cross- Presentation, Current Drug Delivery 2015; 12 (3) . https://dx.doi.org/10.2174/1567201812666141210114747