Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples

Title:Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples

Volume: 10 Issue: 2

Author(s): Mario Contin, Sabrina Flor, Silvia Lucangioli and Valeria Tripodi

Affiliation:

Keywords: Estrogens, molecular imprinting, solid phase extraction, serum, capillary electrophoresis.

Abstract: An estrogen selected molecular imprinted polymer (MIP) has been developed to be used as an alternative sorbent in solid phase extraction of serum samples before capillary electrophoresis analysis. Following a heat polymerization, MIP has been synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker, benzoyl peroxide as radical initiator and 17β- estradiol as template, and toluene as porogen. The optimized molecular imprinted solid phase extraction (MISPE) was compared to traditional C18 solid phase extraction to purify estrogens from complex matrices. It was concluded that the MISPE system developed is useful for the selective extraction of 17β- estradiol and its major natural related estrogens, estriol and estrone, from serum samples with minimal interferences and high recoveries.

Cite this article as:

Contin Mario, Flor Sabrina, Lucangioli Silvia and Tripodi Valeria, Molecularly Imprinted Solid Phase Extraction Before Capillary Electrophoresis for the Analysis of Estrogens in Serum Samples, Current Analytical Chemistry 2014; 10 (2) . https://dx.doi.org/10.2174/15734110113090990027