Abstract
A bioflocculant - producing bacterium was isolated from water samples collected from the Vaigai river and was identified as Klebsiella sp. by morphological and biochemical characteristics. Chemical analysis of the purified bioflocculant indicated that it was a sugar-protein derivative, composed of sugar (76.83%, w/w) and protein (23.17%, w/w). Infrared spectrophotometry revealed that the bioflocculant contains carboxyl, hydroxyl and amino groups as structural components. Scanning electron microscopy (SEM) of the purified bioflocculant showed that it has an irregular, coarse-grained structure connected in netted textures. The concentration of bioflocculant produced after 48 h of cultivation was 0.73 g/L. At the optimal concentration, the bioflocculant caused 91.28% more settling of kaolin than the control in 5 min.
Keywords: Bioflocculant; Klebsiella sp., Flocculating activity, Biochemical Analysis, FTIR, SEM, Kaolin
Current Biotechnology
Title:Production and Characterization of a Novel Bioflocculant from Klebsiella sp.
Volume: 2 Issue: 1
Author(s): L. Muthulakshmi and H. Nellaiah
Affiliation:
Keywords: Bioflocculant; Klebsiella sp., Flocculating activity, Biochemical Analysis, FTIR, SEM, Kaolin
Abstract: A bioflocculant - producing bacterium was isolated from water samples collected from the Vaigai river and was identified as Klebsiella sp. by morphological and biochemical characteristics. Chemical analysis of the purified bioflocculant indicated that it was a sugar-protein derivative, composed of sugar (76.83%, w/w) and protein (23.17%, w/w). Infrared spectrophotometry revealed that the bioflocculant contains carboxyl, hydroxyl and amino groups as structural components. Scanning electron microscopy (SEM) of the purified bioflocculant showed that it has an irregular, coarse-grained structure connected in netted textures. The concentration of bioflocculant produced after 48 h of cultivation was 0.73 g/L. At the optimal concentration, the bioflocculant caused 91.28% more settling of kaolin than the control in 5 min.
Export Options
About this article
Cite this article as:
Muthulakshmi L. and Nellaiah H., Production and Characterization of a Novel Bioflocculant from Klebsiella sp., Current Biotechnology 2013; 2 (1) . https://dx.doi.org/10.2174/2211550111302010009
DOI https://dx.doi.org/10.2174/2211550111302010009 |
Print ISSN 2211-5501 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-551X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Gene Expression Profile Classification: A Review
Current Bioinformatics Tackling Chronic Pain and Inflammation through the Purinergic System
Current Medicinal Chemistry Development of Agents that Modulate Protein-Protein Interactions in Membranes
Current Pharmaceutical Design Bioinspired Microchip Nanoporous Interferometric Sensor for Sensing and Biosensing Applications
Micro and Nanosystems Prediction of Inhibition Activity of BET Bromodomain Inhibitors using Grid Search-Based Extreme Learning Machine and Molecular Docking
Letters in Drug Design & Discovery Leptin and the Ob-Receptor as Anti-Obesity Target: Recent In Silico Advances in the Comprehension of the Protein-Protein Interaction and Rational Drug Design of Anti- Obesity Lead Compounds
Current Pharmaceutical Design DNAzymes: From Creation In Vitro to Application In Vivo
Current Pharmaceutical Biotechnology Shedding Light on the Pathophysiology of Preeclampsia-Syndrome in the Era of Cardio-Obstetrics: Role of Inflammation and Endothelial Dysfunction
Current Hypertension Reviews A Promising Anti-Cancer and Anti-Oxidant Agents Based on the Pyrrole and Fused Pyrrole: Synthesis, Docking Studies and Biological Evaluation
Anti-Cancer Agents in Medicinal Chemistry Data Visualization and Feature Selection Methods in Gel-based Proteomics
Current Protein & Peptide Science Disorder in Milk Proteins: Formation, Structure, Function, Isolation and Applications of Casein Phosphopeptides
Current Protein & Peptide Science A Comparison of the Inhibitory Effects of Anti-Cancer Drugs on Thioredoxin Reductase and Glutathione S-Transferase in Rat Liver
Anti-Cancer Agents in Medicinal Chemistry An Overview on the Pharmacokinetics of Quantum Dots
Current Drug Metabolism An Apple Plus a Brazil Nut a Day Keeps the Doctors Away: Antioxidant Capacity of Foods and their Health Benefits
Current Pharmaceutical Design Synthesis and Cytotoxicity of Amino-Pyrazole Derivatives with Preliminary SAR
Letters in Drug Design & Discovery Recent Advances in Fe-MOF Compositions for Biomedical Applications
Current Medicinal Chemistry Targeting 5-Lipoxygenase for Prevention and Treatment of Cancer
Current Enzyme Inhibition Targets and Patented Drugs for Chemotherapy of Chagas Disease in the Last 15 Years-Period
Recent Patents on Anti-Infective Drug Discovery Development of Purine-Scaffold Small Molecule Inhibitors of Hsp90
Current Cancer Drug Targets Synthesis and in vitro Anti-tumor Activity of Some New Sebacoyl Chloride Based Heterocycles
Current Organic Synthesis