Abstract
A bioflocculant - producing bacterium was isolated from water samples collected from the Vaigai river and was identified as Klebsiella sp. by morphological and biochemical characteristics. Chemical analysis of the purified bioflocculant indicated that it was a sugar-protein derivative, composed of sugar (76.83%, w/w) and protein (23.17%, w/w). Infrared spectrophotometry revealed that the bioflocculant contains carboxyl, hydroxyl and amino groups as structural components. Scanning electron microscopy (SEM) of the purified bioflocculant showed that it has an irregular, coarse-grained structure connected in netted textures. The concentration of bioflocculant produced after 48 h of cultivation was 0.73 g/L. At the optimal concentration, the bioflocculant caused 91.28% more settling of kaolin than the control in 5 min.
Keywords: Bioflocculant; Klebsiella sp., Flocculating activity, Biochemical Analysis, FTIR, SEM, Kaolin
Current Biotechnology
Title:Production and Characterization of a Novel Bioflocculant from Klebsiella sp.
Volume: 2 Issue: 1
Author(s): L. Muthulakshmi and H. Nellaiah
Affiliation:
Keywords: Bioflocculant; Klebsiella sp., Flocculating activity, Biochemical Analysis, FTIR, SEM, Kaolin
Abstract: A bioflocculant - producing bacterium was isolated from water samples collected from the Vaigai river and was identified as Klebsiella sp. by morphological and biochemical characteristics. Chemical analysis of the purified bioflocculant indicated that it was a sugar-protein derivative, composed of sugar (76.83%, w/w) and protein (23.17%, w/w). Infrared spectrophotometry revealed that the bioflocculant contains carboxyl, hydroxyl and amino groups as structural components. Scanning electron microscopy (SEM) of the purified bioflocculant showed that it has an irregular, coarse-grained structure connected in netted textures. The concentration of bioflocculant produced after 48 h of cultivation was 0.73 g/L. At the optimal concentration, the bioflocculant caused 91.28% more settling of kaolin than the control in 5 min.
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Cite this article as:
Muthulakshmi L. and Nellaiah H., Production and Characterization of a Novel Bioflocculant from Klebsiella sp., Current Biotechnology 2013; 2 (1) . https://dx.doi.org/10.2174/2211550111302010009
DOI https://dx.doi.org/10.2174/2211550111302010009 |
Print ISSN 2211-5501 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-551X |
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