Application of In-Line Liquid Chromatography-Accurate Radioisotope Counting-Mass Spectrometry (LC-ARC-MS) to Evaluate Metabolic Profile of [3H]-Mefenamic Acid in Rat Plasma

Title: Application of In-Line Liquid Chromatography-Accurate Radioisotope Counting-Mass Spectrometry (LC-ARC-MS) to Evaluate Metabolic Profile of [3H]-Mefenamic Acid in Rat Plasma

Volume: 1 Issue: 3

Author(s): Wing Lam, Cho-Ming Loi, James Atherton, Wayne Stolle, John Easter and Abdul Mutlib

Affiliation:

Keywords: Mefenamic acid metabolism, sulfate, glucuronide, LC-ARC-MS, plasma

Abstract: Profiling of rat plasma using a highly sensitive LC-ARC-MS technique showed that [3H] mefenamic acid was metabolized to several products, including a sulfate conjugate and a hydroxylated analogue as major metabolites. This technique of detecting low levels of radioactivity in plasma was superior to previously used methods, such as β-RAM detectors.

Cite this article as:

Wing Lam , Cho-Ming Loi , James Atherton , Wayne Stolle , John Easter and Abdul Mutlib , Application of In-Line Liquid Chromatography-Accurate Radioisotope Counting-Mass Spectrometry (LC-ARC-MS) to Evaluate Metabolic Profile of [3H]-Mefenamic Acid in Rat Plasma, Drug Metabolism Letters 2007; 1 (3) . https://dx.doi.org/10.2174/187231207781369771