Login Register Cart 0
Generic placeholder image

Current Bioactive Compounds

Editor-in-Chief

ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Role of Quercetin in the Mitigation of Lindane Induced Toxicity in Terms of Oxidative Responses and Metabolic Status in Mice Brain

Author(s): Anupama Sharma, Renu Bist* and Hemant Pareek

Volume 17, Issue 7, 2021

Published on: 23 November, 2020

Article ID: e010621188266 Pages: 10

DOI: 10.2174/1573407216999201123193457

Price: $65

Abstract

Background: The current study evaluated the protective potential of quercetin against lindane induced toxicity in mice brain. For investigation, mice were allocated into four groups; the first group was the control; the second group was administered with an oral dose of lindane (25 mg/kg bw) for 4 consecutive days; the third group was exposed to quercetin (40 mg/kg bw) and to the fourth group, quercetin was administered 1 hour prior to the exposure of lindane.

Objective: Two major objectives of the study were set . The first objective was to create lesions in the brain by lindane and; the second was to evaluate the neuroprotective potential of quercetin.

Methods: To study oxidative responses, the levels of thiobarbituric Acid Reactive Substances (TBARS), Protein Carbonyl Content (PCC), Reduced Glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione Peroxidase (GPx) were measured in brain homogenates. Three key steps regulating enzymes of the Tricarboxylic Acid (TCA) cycle viz citrate synthase (CS), pyruvate dehydrogenase (PDH) and fumarase were also assayed.

Results: Lindane treatment significantly enhanced the levels of TBARS (P<0.001),PCC (P<0.001), GPx (P<0.001), SOD (P<0.05), PDH (P<0.05) and fumarase (P<0.001) in brains of mice compared to control. Meanwhile, it alleviated GSH, CAT and CS (P<0.05) activity.

Conclusion: Pretreatment with quercetin in lindane treated group not only restored previously altered biochemical parameters after lindane treatment and also significantly improved them, which suggests that quercetin is not only safe rather is neuroprotective against lindane intoxication.

Keywords: Lindane, quercetin, oxidative stress parameters, TCA cycle enzymes, neuroprotective, stroke.

Graphical Abstract

[1]
Costabeber I, Trindade R, Fries LM. Organochlorine pesticides level in cow milk. Alimentaria 2001; 38: 127-9.
[2]
Schinas V, Leotsinidis M, Alexopoulos A, Tsapanos V, Kondakis XG. Organochlorine pesticide residues in human breast milk from southwest Greece: associations with weekly food consumption patterns of mothers. Arch Environ Health 2000; 55(6): 411-7.
[http://dx.doi.org/10.1080/00039890009604039] [PMID: 11128879]
[3]
Bist R, Bhatt DK. The evaluation of effect of alpha-lipoic acid and vitamin E on the lipid peroxidation, gamma-amino butyric acid and serotonin level in the brain of mice (Mus musculus) acutely intoxicated with lindane. J Neurol Sci 2009; 276(1-2): 99-102.
[http://dx.doi.org/10.1016/j.jns.2008.09.008] [PMID: 18950802]
[4]
Bist R, Misra S, Bhatt DK. Inhibition of lindane-induced toxicity using alpha-lipoic acid and vitamin E in the brain of Mus musculus. Protoplasma 2010; 242(1-4): 49-53.
[http://dx.doi.org/10.1007/s00709-010-0121-0] [PMID: 20490610]
[5]
Bist R, Behera KK, Bhatt DK. Effect of an antioxidant combination on the distribution of acetylcholinesterase and adenosine triphosphatase activities in the cerebellum of in lindane-intoxicated mice. J Exp Integr Med 2013; 3(2): 103-12.
[http://dx.doi.org/10.5455/jeim.080413.or.066]
[6]
Skaper SD, Floreani M, Ceccon M, Facci L, Giusti P. Excitotoxicity, oxidative stress, and the neuroprotective potential of melatonin. Ann N Y Acad Sci 1999; 890(1): 107-18.
[http://dx.doi.org/10.1111/j.1749-6632.1999.tb07985.x] [PMID: 10668417]
[7]
Chaudhary B, Agarwal S, Bist R. Invulnerability of bromelain against oxidative degeneration and cholinergic deficits imposed by dichlorvos in mice brains. Front Biol (Beijing) 2018; 13(1): 1674-7992.
[http://dx.doi.org/10.1007/s11515-018-1479-1]
[8]
Bondy SC. The relation of oxidative stress and hyperexcitation to neurological disease. Proc Soc Exp Biol Med 1995; 208(4): 337-45.
[http://dx.doi.org/10.3181/00379727-208-43862] [PMID: 7700883]
[9]
Agarwal S, Chaudhary B, Bist R. Bacoside A and bromelain relieve dichlorvos induced changes in oxidative responses in mice serum. Chem Biol Interact 2016; 254: 173-8.
[http://dx.doi.org/10.1016/j.cbi.2016.05.017] [PMID: 27180203]
[10]
Halliwell B, Gutteridge JMC. Free radicals, lipid peroxidation, and cell damage. Lancet 1984; 2(8411): 1095.
[http://dx.doi.org/10.1016/S0140-6736(84)91530-7] [PMID: 6150163]
[11]
Agarwal S, Chaudhary B, Bist R. Protective propensity of bacoside A and bromelain on renal cholinesterases, γ-Aminobutyric acid and serotonin level of Mus musculus intoxicated with dichlorvos. Chem Biol Interact 2017; 261: 139-44.
[http://dx.doi.org/10.1016/j.cbi.2016.11.027] [PMID: 27899289]
[12]
Chavko M, Harabin AL. Regional lipid peroxidation and protein oxidation in rat brain after hyperbaric oxygen exposure. Free Radic Biol Med 1996; 20(7): 973-8.
[http://dx.doi.org/10.1016/0891-5849(95)02181-7] [PMID: 8743983]
[13]
Chelikani P, Fita I, Loewen PC. Diversity of structures and properties among catalases. Cell Mol Life Sci 2004; 61(2): 192-208.
[http://dx.doi.org/10.1007/s00018-003-3206-5] [PMID: 14745498]
[14]
Ansari MA, Abdul HM, Joshi G, Opii WO, Butterfield DA. Protective effect of quercetin in primary neurons against Abeta(1-42): relevance to Alzheimer’s disease. J Nutr Biochem 2009; 20(4): 269-75.
[http://dx.doi.org/10.1016/j.jnutbio.2008.03.002] [PMID: 18602817]
[15]
Lakhanpal P, Rai DK. Quercetin: a versatile flavonoid. Internet Journal of Medical Update 2007; 2(2): 22-37.
[16]
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-8.
[http://dx.doi.org/10.1016/0003-2697(79)90738-3] [PMID: 36810]
[17]
Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7(2): 88-95.
[http://dx.doi.org/10.1016/0006-2952(61)90145-9] [PMID: 13726518]
[18]
Levine RL, Garland D, Oliver CN, et al. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 1990; 186: 464-78.
[http://dx.doi.org/10.1016/0076-6879(90)86141-H] [PMID: 1978225]
[19]
Rossi R, Milzani A, Dalle-Donne I, et al. Blood glutathione disulfide: in vivo factor or in vitro artifact? Clin Chem 2002; 48(5): 742-53.
[http://dx.doi.org/10.1093/clinchem/48.5.742] [PMID: 11978601]
[20]
Dhindsa RS, Plumb-Dhindsa P, Thorpe TA. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 1981; 32(1): 93-101.
[http://dx.doi.org/10.1093/jxb/32.1.93]
[21]
Claiborne A. Catalase activity.CRC handbook of methods for oxygen radical research. Florida: CRC Press 1985; pp. 283-4.
[22]
Mohandas J, Marshall JJ, Duggin GG, Horvath JS, Tiller DJ. Differential distribution of glutathione and glutathione-related enzymes in rabbit kidney. Possible implications in analgesic nephropathy. Biochem Pharmacol 1984; 33(11): 1801-7.
[http://dx.doi.org/10.1016/0006-2952(84)90353-8] [PMID: 6145422]
[23]
Gibson GE, Sheu KF, Blass JP. Abnormalities of mitochondrial enzymes in Alzheimer disease. J Neural Transm (Vienna) 1998; 105(8-9): 855-70.
[http://dx.doi.org/10.1007/s007020050099] [PMID: 9869323]
[24]
Shepherd D, Garland PB. The kinetic properties of citrate synthase from rat liver mitochondria. Biochem J 1969; 114(3): 597-610. [EC 4.1. 3.7 Citrate oxaloacetage-lyase (CoA-acetylating)].
[http://dx.doi.org/10.1042/bj1140597] [PMID: 5820645]
[25]
Hill RL, Bradshaw RA. Fumarase In: Lowenstein JM, Ed. Methods in Enzymology. 1969; 13: pp. 91-2.
[26]
WHO. Lindane. Environmental Health Criteria 124. World Health Organization. Geneva Wiley & Sons 1991; 5: pp. 267-81.
[27]
Antunes-Madeira MC, Almeida LM, Madeira VMC. Depth-dependent effects of DDT and lindane on the fluidity of native membranes and extracted lipids. Implications for mechanisms of toxicity. Bull Environ Contam Toxicol 1993; 51(6): 787-94.
[http://dx.doi.org/10.1007/BF00198271] [PMID: 7504961]
[28]
Dobretsov GE, Borschevskaya TA, Petrov VA, Vladimirov YA. The increase of phospholipid bilayer rigidity after lipid peroxidation. FEBS Lett 1977; 84(1): 125-8.
[http://dx.doi.org/10.1016/0014-5793(77)81071-5] [PMID: 590513]
[29]
Alsharif NZ, Lawson T, Stohs SJ. Oxidative stress induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin is mediated by the aryl hydrocarbon (Ah) receptor complex. Toxicology 1994; 92(1-3): 39-51.
[http://dx.doi.org/10.1016/0300-483X(94)90166-X] [PMID: 7940568]
[30]
Halliwell BE. Lipid peroxidation: a radical chain reaction Free radicals in Biology and Medicine 1989; 188-276.
[31]
Hincal F, Gürbay A, Giray B. Induction of lipid peroxidation and alteration of glutathione redox status by endosulfan. Biol Trace Elem Res 1995; 47(1-3): 321-6.
[http://dx.doi.org/10.1007/BF02790133] [PMID: 7779565]
[32]
Junqueira VB, Simizu K, Van Halsema L, Koch OR, Barros SB, Videla LA. Lindane-induced oxidative stress. I. Time course of changes in hepatic microsomal parameters, antioxidant enzymes, lipid peroxidative indices and morphological characteristics. Xenobiotica 1988; 18(11): 1297-304.
[http://dx.doi.org/10.3109/00498258809042253] [PMID: 2469257]
[33]
Stadtman ER, Levine RL. Chemical modification of proteins by reactive oxygen species Redox proteomics: from protein modifications to cellular dysfunction and diseases 2006; 9(3): 293-304.
[34]
Kosower NS, Kosower EM. The glutathione status of cells. Int Rev Cytol 1978; 54: 109-60.
[http://dx.doi.org/10.1016/S0074-7696(08)60166-7] [PMID: 42630]
[35]
Bano M, Bhatt DK. Neuroprotective role of a novel combination of certain antioxidants on lindane (g-HCH) induced toxicity in cerebrum of mice. Res J Agric Biol Sci 2007; 3(6): 664-9.
[36]
Tsang EW, Bowler C, Hérouart D, et al. Differential regulation of superoxide dismutases in plants exposed to environmental stress. Plant Cell 1991; 3(8): 783-92.
[PMID: 1820818]
[37]
Padma VV, Baskaran R, Roopesh RS, Poornima P. Quercetin attenuates lindane induced oxidative stress in Wistar rats. Mol Biol Rep 2012; 39(6): 6895-905.
[http://dx.doi.org/10.1007/s11033-012-1516-0] [PMID: 22302394]
[38]
Danson MJ, Hough DW. Citrate synthase from hyperthermophilic Archaea. Methods Enzymol 2001; 331: 3-12. [EC 4.1. 3.7. Citrate oxaloacetate-lyase (CoA-acetylating)].
[http://dx.doi.org/10.1016/S0076-6879(01)31042-X] [PMID: 11265473]
[39]
Jessup W, Dean RT, de Whalley CV, Rankin SM, Leake DS. The role of oxidative modification and antioxidants in LDL metabolism and atherosclerosis. Adv Exp Med Biol 1990; 264: 139-42.
[http://dx.doi.org/10.1007/978-1-4684-5730-8_20] [PMID: 2173869]
[40]
Chopra M, Fitzsimons PEE, Strain JJ, Thurnham DI, Howard AN. Nonalcoholic red wine extract and quercetin inhibit LDL oxidation without affecting plasma antioxidant vitamin and carotenoid concentrations. Clin Chem 2000; 46(8 Pt 1): 1162-70.
[http://dx.doi.org/10.1093/clinchem/46.8.1162] [PMID: 10926898]

Rights & Permissions Print Cite
Article Metrics
5
1
© 2024 Bentham Science Publishers | Privacy Policy