摘要
碱溶性多糖(ASP)是从四曲霉菌(烟曲霉,构巢曲霉,A. niger, 和 A. wentii)细胞壁分离所得。化学和光谱研究(免疫荧光标记,组成分析,甲基化分析,FTIR,1H NMR)表明,ASPs几乎完全由聚合物(1→3)-α-D-脱氧葡萄糖组成。(1→3)-α-D-葡聚糖羧甲基化后(CM),其活性在人类三大细胞系(HSF,HeLa, Jurkat)中进行评估。CM-α-D-葡聚糖在抗增殖,细胞毒性,和清除自由基等方面的作用进行了分析。所有测试的CM-α-D-葡聚糖细胞代谢作用减弱。然而,从A. wentii和A. niger中分离所得的CM-α-D-葡聚糖可增加(约50%)HSF细胞活力。此外,从烟曲霉A、构巢曲霉A中培养所得的CM-α-D-葡聚糖发现其活力可增至5倍以上。从检测曲霉菌的结果表示,CM-(1→3)-α-D-葡聚糖没有清除自由基的作用。荧光染色法显示CM-α-D-葡聚糖对细胞活力产生轻微的毒性作用,而对细胞骨架组织的F-肌动蛋白无影响。
关键词: (1→3)-α-D-葡聚糖,羧甲基化,黑曲霉,细胞毒素,人细胞培养物
图形摘要
Current Drug Targets
Title:(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives
Volume: 16 Issue: 13
Author(s): Adrian Wiater, Roman Paduch, Adam Choma, Staczek Sylwia, Małgorzata Pleszczynska, Michal Tomczyk, Marcello Locatelli and Szczodrak Janusz
Affiliation:
关键词: (1→3)-α-D-葡聚糖,羧甲基化,黑曲霉,细胞毒素,人细胞培养物
摘要: Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii). The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose. After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed. Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans was analysed. All the tested CM-α-D-glucans decreased cellular metabolism. However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells. Moreover, an over 5-fold increase in the viability was found for Jurkat cells incubated with CM-α-D-glucans from A. fumigatus and A. nidulans. The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action. Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
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Adrian Wiater, Roman Paduch, Adam Choma, Staczek Sylwia, Małgorzata Pleszczynska, Michal Tomczyk, Marcello Locatelli and Szczodrak Janusz , (1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives, Current Drug Targets 2015; 16 (13) . https://dx.doi.org/10.2174/1389450116666150120105133
DOI https://dx.doi.org/10.2174/1389450116666150120105133 |
Print ISSN 1389-4501 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5592 |
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