Affinity-Based Methods in Drug-Target Discovery

Title:Affinity-Based Methods in Drug-Target Discovery

Volume: 16 Issue: 1

Author(s): Gabriela Rylova, Tomas Ozdian, Lakshman Varanasi, Miroslav Soural, Jan Hlavac, Dusan Holub, Petr Dzubak and Marian Hajduch

Affiliation:

Keywords: Affinity purification, drug-target discovery, immobilization, mass-spectrometry, probe, quantification.

Abstract: Target discovery using the molecular approach, as opposed to the more traditional systems approach requires the study of the cellular or biological process underlying a condition or disease. The approaches that are employed by the “bench” scientist may be genetic, genomic or proteomic and each has its rightful place in the drug-target discovery process. Affinity-based proteomic techniques currently used in drug-discovery draw upon several disciplines, synthetic chemistry, cell-biology, biochemistry and mass spectrometry. An important component of such techniques is the probe that is specifically designed to pick out a protein or set of proteins from amongst the varied thousands in a cell lysate. A second component, that is just as important, is liquid-chromatography tandem massspectrometry (LC-MS/MS). LC-MS/MS and the supporting theoretical framework has come of age and is the tool of choice for protein identification and quantification. These proteomic tools are critical to maintaining the drug-candidate supply, in the larger context of drug discovery.

Cite this article as:

Rylova Gabriela, Ozdian Tomas, Varanasi Lakshman, Soural Miroslav, Hlavac Jan, Holub Dusan, Dzubak Petr and Hajduch Marian, Affinity-Based Methods in Drug-Target Discovery, Current Drug Targets 2015; 16 (1) . https://dx.doi.org/10.2174/1389450115666141120110323