Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute

Title:Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute

Volume: 17 Issue: 4

Author(s): Emma J. Shanks

Affiliation:

Keywords: Cancer, high content analysis, phenotypic screening, RNAi screening, statistical analysis.

Abstract: The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.

Cite this article as:

Shanks J. Emma, Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute, Combinatorial Chemistry & High Throughput Screening 2014; 17 (4) . https://dx.doi.org/10.2174/1386207317666140323133249