Genistein Inhibits Cell Growth and Induces Apoptosis Through Up-regulation of miR-34a in Pancreatic Cancer Cells

Title:Genistein Inhibits Cell Growth and Induces Apoptosis Through Up-regulation of miR-34a in Pancreatic Cancer Cells

Volume: 13 Issue: 14

Author(s): Jun Xia, Qiaoling Duan, Aamir Ahmad, Bin Bao, Sanjeev Banerjee, Ying Shi, Jia Ma, Jian Geng, Zhiwen Chen, KM Wahidur Rahman, Lucio Miele, Fazlul H Sarkar and Zhiwei Wang

Affiliation:

Keywords: Apoptosis, cell growth, genistein, miR-34a, Notch-1, pancreatic cancer

Abstract: Pancreatic cancer (PC) is the fourth most common cause of cancer-related deaths in the United States, suggesting that designing novel therapeutic strategy is required to improve the survival outcome of patients diagnosed with PC. Recently, microRNAs (miRNA) have been found to be involved in the regulation of multiple aspects of tumor development and progression including PC. In this study, we investigate whether miR-34a plays a critical role in the control of cell growth and apoptosis in PC cells. We found that Re-expression (forced expression) of miR-34a inhibits cell growth and induces apoptosis, with concomitant down-regulation of Notch-1 signaling pathway, one of the target of miR-34a. Moreover, treatment of PC cells with a natural compound genistein led to the up-regulation of miR-34a, resulting in the down-regulation of Notch-1, which was correlated with inhibition of cell growth, and induction of apoptosis. Our findings suggest that genistein could function as a non-toxic activator of a miRNA that can suppress the proliferation of PC cells.

Cite this article as:

Xia Jun, Duan Qiaoling, Ahmad Aamir, Bao Bin, Banerjee Sanjeev, Shi Ying, Ma Jia, Geng Jian, Chen Zhiwen, Wahidur Rahman KM, Miele Lucio, H Sarkar Fazlul and Wang Zhiwei, Genistein Inhibits Cell Growth and Induces Apoptosis Through Up-regulation of miR-34a in Pancreatic Cancer Cells, Current Drug Targets 2012; 13 (14) . https://dx.doi.org/10.2174/138945012804545597