Making the Most of Pathological Specimens: Molecular Diagnosis in Formalin-Fixed, Paraffin Embedded Tissue

Title:Making the Most of Pathological Specimens: Molecular Diagnosis in Formalin-Fixed, Paraffin Embedded Tissue

Volume: 13 Issue: 12

Author(s): Jennifer A. Fairley, Katelyn Gilmour and Kathy Walsh

Affiliation:

Keywords: Biomarkers, BRAF, EGFR, FFPE tissue, FFPE diagnostics, HER2, KRAS, molecular diagnostics, molecular pathology, personalised medicine, targeted therapy.

Abstract: The development of commercial reagents designed specifically for use with formalin-fixed paraffin-embedded (FFPE) tissue has unlocked the diagnostic potential of this prolific resource. The availability of archival FFPE tissue and tissue from current patients make it an ideal resource for molecular testing. Despite its stability and ability to preserve morphological information, FFPE provides a number of technical challenges to the study of biomolecules. In particular, the cross-linking and processing present problems in the extraction and isolation of DNA, RNA and protein and affect their use in downstream analysis. Here we will discuss some of the problems of FFPE tissue, how they can be overcome and how FFPE material can be used within clinical molecular diagnostics.

Cite this article as:

A. Fairley Jennifer, Gilmour Katelyn and Walsh Kathy, Making the Most of Pathological Specimens: Molecular Diagnosis in Formalin-Fixed, Paraffin Embedded Tissue, Current Drug Targets 2012; 13 (12) . https://dx.doi.org/10.2174/138945012803530125