A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells

Title: A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells

Volume: 9 Issue: 10

Author(s): Chia-Jung Chen, Chia Che Tsai, Jung-Feng Hsieh, Chichen Michael Chien, Tzu-Hua Wu and Shui-Tein Chen

Affiliation:

Keywords: High-throughput screening, human umbilical cord blood mononuclear cells, natural products, flow cytometry, immuno-regulatory activities, γ-dodecalactone

Abstract: A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and - dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.

Cite this article as:

Chen Chia-Jung, Che Tsai Chia, Hsieh Jung-Feng, Michael Chien Chichen, Wu Tzu-Hua and Chen Shui-Tein, A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells, Combinatorial Chemistry & High Throughput Screening 2006; 9 (10) . https://dx.doi.org/10.2174/138620706779026033