Kinetics of Interaction of HLA-B2705 with Natural Killer Cell Immunoglobulin- Like Receptor 3DS1

Title: Kinetics of Interaction of HLA-B2705 with Natural Killer Cell Immunoglobulin- Like Receptor 3DS1

Volume: 17 Issue: 5

Author(s): Hui Li, Shun-Lin Peng, Yu Cui, Qiu-Xia Fu, Yong Zhou, Quan-Li Wang, Lin-Sheng Zhan and Sen Zhong

Affiliation:

Keywords: HLA-B2705, peptide, Killer cell immunoglobulin-like receptor, prokaryotic expression, purification, SPR, BIAcore, affinity

Abstract: The recognition of human leukocyte antigen (HLA) molecules by specific receptors is a crucial step in the regulation of natural killer (NK) cell function. Killer cell immunoglobulin-like receptor (KIR) 3DS1 is one of the activating receptors of NK cell and is implicated in slowing disease progression in HIV infection. KIR3DS1 play an important role in the outcome of multiple diseases associated with viral infections. In contrast to the inhibitory receptor, much less is known about the ligands of KIR3DS1. In order to achieve a better understanding of the biology of KIR3DS1 and its ligand systems, it is necessary to identify the ligands of KIR3DS1. In this work, we utilized recombinant HLA-B2705 molecules and DsbA-KIR3DS1 fusion protein to monitor the interaction between HLA-B2705 complexes and DsbAKIR3DS1 using BIAcore 3000 SPR sensor and found that the specific binding between KIR3DS1 and HLA-B2705 existed and the affinity was 6.95×10-6 mol//L. So we concluded that HLA-B2705 is a possible ligand of KIR3DS1.

Cite this article as:

Li Hui, Peng Shun-Lin, Cui Yu, Fu Qiu-Xia, Zhou Yong, Wang Quan-Li, Zhan Lin-Sheng and Zhong Sen, Kinetics of Interaction of HLA-B2705 with Natural Killer Cell Immunoglobulin- Like Receptor 3DS1, Protein & Peptide Letters 2010; 17 (5) . https://dx.doi.org/10.2174/092986610791112657