Pomegranin, An Antifungal Peptide from Pomegranate Peels

Title: Pomegranin, An Antifungal Peptide from Pomegranate Peels

Volume: 16 Issue: 1

Author(s): Guang Guo, He Xiang Wang and Tzi Bun Ng

Affiliation:

Keywords: antifungal peptide, pomegranate, isolation, peel

Abstract: A new antifungal peptide designated as pomegranin, with an N-terminal sequence resembling that of rice disease resistance NB-S-LRR-like protein, was isolated from fresh pomegranate peels by ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Pomegranin was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. It exhibited a molecular mass of 11 kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It inhibited mycelial growth in the fungi Botrytis cinerea and Fusarium oxysporum with an IC50 of 2 μM and 6.1 μM, respectively. It was devoid of hemagglutinating, ribonuclease, deoxyribonuclease and protease inhibitory activities.

Cite this article as:

Guo Guang, Wang Xiang He and Ng Bun Tzi, Pomegranin, An Antifungal Peptide from Pomegranate Peels, Protein & Peptide Letters 2009; 16 (1) . https://dx.doi.org/10.2174/092986609787049330