Abstract
The study explores in vitro by circular dichroism and mass spectrometry the effects of pH, Cu+2 ions and sheetbreakers on the secondary structures and self-aggregation of b-amyloid peptides [Aβ43, Aβ42 and Ab40] of Alzheimers disease. Within pH 5.4-7.3, more sheet structures and aggregates containing up to 11 peptide units were observed. Cu+2 ions led to oxidative degradation or aggregation depending on its concentration and time of incubation. β-sheet breakers can reverse the self-aggregation process, suggesting their potential therapeutic use.
Keywords: amyloid peptides, protein aggregation, secondary structure, sheet breakers, copper ions, ph, circular dichroism, mass spectrometry
Protein & Peptide Letters
Title: Modulatory Effects of pH, Cu+2 and Sheet Breakers on Aggregation of Amyloid Peptides
Volume: 12 Issue: 2
Author(s): Kris Beking, Xiaolei Hao, Sarmistha Basak and Ajoy Basak
Affiliation:
Keywords: amyloid peptides, protein aggregation, secondary structure, sheet breakers, copper ions, ph, circular dichroism, mass spectrometry
Abstract: The study explores in vitro by circular dichroism and mass spectrometry the effects of pH, Cu+2 ions and sheetbreakers on the secondary structures and self-aggregation of b-amyloid peptides [Aβ43, Aβ42 and Ab40] of Alzheimers disease. Within pH 5.4-7.3, more sheet structures and aggregates containing up to 11 peptide units were observed. Cu+2 ions led to oxidative degradation or aggregation depending on its concentration and time of incubation. β-sheet breakers can reverse the self-aggregation process, suggesting their potential therapeutic use.
Export Options
About this article
Cite this article as:
Beking Kris, Hao Xiaolei, Basak Sarmistha and Basak Ajoy, Modulatory Effects of pH, Cu+2 and Sheet Breakers on Aggregation of Amyloid Peptides, Protein & Peptide Letters 2005; 12 (2) . https://dx.doi.org/10.2174/0929866053005845
DOI https://dx.doi.org/10.2174/0929866053005845 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
A Novel Prescription for Alzheimer’s Disease: Targeting Hypercoagulable States
Current Neurovascular Research The Cholinergic Potential, the Vagus Nerve and Challenges in Treatment of Traumatic Brain Injury
Current Pharmaceutical Design Dual Functional Small Molecule Probes as Fluorophore and Ligand for Misfolding Proteins
Current Organic Chemistry Optimizing ADAS-Cog Worksheets: A Survey of Clinical Trial Rater s’ Perceptions
Current Alzheimer Research New Therapeutic Strategies for Cancer and Neurodegeneration Emerging from Yeast Cell-based Systems
Current Pharmaceutical Design Alzheimers Disease and Retinal Neurodegeneration
Current Alzheimer Research Data-driven Approach to Detect and Predict Adverse Drug Reactions
Current Pharmaceutical Design Structural Determinants of the Multifunctional Profile of Dual Binding Site Acetylcholinesterase Inhibitors as Anti-Alzheimer Agents
Current Pharmaceutical Design Primary Progressive Aphasia-Defining Genetic and Pathological Subtypes
Current Alzheimer Research Strategies to Diminish the Ab Load in Alzheimers Disease
Current Medicinal Chemistry - Central Nervous System Agents Meet Our Editorial Board Member:
Cardiovascular & Hematological Agents in Medicinal Chemistry Protective Effect of Aspirin Against Oligomeric Aβ42 Induced Mitochondrial Alterations and Neurotoxicity in Differentiated EC P19 Neuronal Cells
Current Alzheimer Research Learning from the Past: A Review of Clinical Trials Targeting Amyloid, Tau and Neuroinflammation in Alzheimer’s Disease
Current Alzheimer Research Patent Selections
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery (Discontinued) Editorial [Hot Topic: Ischemic Stroke in Very Old Patients (Guest Editor: Maria Cristina Zurru)]
Cardiovascular & Hematological Disorders-Drug Targets A Second Look into the Oxidant Mechanisms in Alzheimers Disease
Current Neurovascular Research Dexmedetomidine Use in General Anaesthesia
Current Drug Targets Vitamin D and Cardiovascular Disease
Current Vascular Pharmacology May Endocrine Therapy be Associated with Cognitive Impairment in Breast Cancer Patients?
Clinical Cancer Drugs Music Therapy for Individuals with Dementia: Areas of Interventions and Research Perspectives
Current Alzheimer Research