Crystallization and Preliminary Crystallographic Studies of an Antitumour Lectin from the Edible Mushroom Agrocybe aegerita

Title: Crystallization and Preliminary Crystallographic Studies of an Antitumour Lectin from the Edible Mushroom Agrocybe aegerita

Volume: 12 Issue: 7

Author(s): Na Yang, Yi Liang, Ye Xiang, Ying Zhang, Hui Sun and Da-Cheng Wang

Affiliation:

Keywords: lectin, mushroom, antitumour, apoptosis, dnase activity, crystallization

Abstract: An antitumour lectin named AAL has been purified from the fruiting body of edible mushroom Agrocybe aegerita. In addition to having a distinct bioactivity, AAL shows strong inhibition effects on human and mouse tumour cells. It has been shown that AAL exerts its antitumour effects via apoptosis-induction. AAL and AAL-lactose complex have been crystallized and their diffraction data were collected with resolution of 2.6 Å and 3.0 Å, respectively. Both crystals belong to space group P 6122 with unit cell parameters a = 123.98 Å, b = 123.98 Å, c = 56.86 Å, a= b=90 , g = 120 and a = 123.69 Å, b = 123.69 Å, c = 56.64 Å, α= β=90 , γ = 120 , respectively.

Cite this article as:

Yang Na, Liang Yi, Xiang Ye, Zhang Ying, Sun Hui and Wang Da-Cheng, Crystallization and Preliminary Crystallographic Studies of an Antitumour Lectin from the Edible Mushroom Agrocybe aegerita, Protein & Peptide Letters 2005; 12 (7) . https://dx.doi.org/10.2174/0929866054696181