Abstract
Background: Eremurus spectabilis BIEB. (Liliaceae) is an edible and medicinal plant in Turkey.
Introduction: This study was designed to isolate and quantify isoorientin in leaves of E. spectabilis and exhibit its effect on SH-SY5Y neuroblastoma cell line.
Methods: Purity and identification of isoorientin were evaluated by 1D-NMR, 2D-NMR and Q-TOF were isolated from E. spectabilis via column chromatography. An HPLC method was also developed and validated for isoorientin.
Results: Quantitative measurements indicated that contents of isoorientin in E. spectabilis leaves were 81.01 mg/g and MeOH extract were 23.75 mg/gr. All measurements were performed at 350 nm. Anticancer activity was investigated on cell culture. IC50 doses of isoorientin were detected as 250 µM at the 48th hour in SH-SY5Y cells by XTT assay. Real-time PCR analysis in SH-SY5Y cells showed that CCND1, CDK6, casp-9, Bax, ATR, Bcl-2, CHEK1 and CHEK2, expressions significantly reduced in experimental group when compared with the control group. p53, p21, caspase-3, caspase-8, Bcl-2, ATM and ERCC1 expressions increased in the experimental group when compared with the control group (P <0.05).
Conclusion: Measurements revealed that E. spectabilis contains high amount of isoorientin and can be used as a new source of this compound. In addition to this, isoorientin affects cell proliferation of neuroblastoma cells by cell cycle control and apoptosis gene expression and it could be used as a therapeutic agent, however, more studies must be performed to clarify its mechanism.
Keywords: Eremurus spectabilis BIEB., HPLC, isoorientin, neuroblastoma, isolation, XTT.
Graphical Abstract
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