LC/MS/MS Profiling of Tissue Oxysterols and its Application in Dextran Sodium Sulphate Induced Mouse Colitis Models

Title:LC/MS/MS Profiling of Tissue Oxysterols and its Application in Dextran Sodium Sulphate Induced Mouse Colitis Models

Volume: 17 Issue: 24

Author(s): Jiejun Wu*, J.Eric McDuffie*, Jiao Song, Michael C. Harris, Holly Raymond, Yafei Chen, Leslie Nguyen, Steven Nguyen, Justin Kanerva, Heather M. McAllister, Xiaohu Deng, Neelakandha Mani, Changlu Liu and Siquan Sun

Affiliation:

• Janssen R&D, LLC., 3210 Merryfield Row, San Diego, CA 92121,United States

• Janssen R&D, LLC., 3210 Merryfield Row, San Diego, CA 92121,United States

Keywords: Oxysterol separation, Oxysterol quantification, Oxysterol analysis, Dihydroxycholesterols, Mouse colitis biomarker.

Abstract: We have developed a workflow to extract, separate, and semi-quantify bioactive oxysterols from mouse colon tissues and fecal matters using solid- and liquid-phase extractions, enzymatic and chemical modifications, and stable-isotope dilution LC/MS/MS. The method was applied to a dextran sodium sulphate (DSS)-induced mouse colitis model, which revealed that one particular dihydroxycholesterol (diOHC), 7α,25-diOHC, was significantly elevated in both colon tissue and fecal matters of mice with colitis compared to that in naïve mice. The extent of 7α,25-diOHC elevation was positively correlated with colitis severity.

Cite this article as:

Wu Jiejun *, McDuffie J.Eric *, Song Jiao , Harris C. Michael , Raymond Holly , Chen Yafei , Nguyen Leslie , Nguyen Steven , Kanerva Justin , McAllister M. Heather , Deng Xiaohu , Mani Neelakandha , Liu Changlu and Sun Siquan , LC/MS/MS Profiling of Tissue Oxysterols and its Application in Dextran Sodium Sulphate Induced Mouse Colitis Models, Current Topics in Medicinal Chemistry 2017; 17 (24) . https://dx.doi.org/10.2174/1568026617666170713165519