Characterization of Mutants of Sulfolobus solfataricus Signature Amidase Able to Hydrolyse R-Ketoprofen Amide

Title: Characterization of Mutants of Sulfolobus solfataricus Signature Amidase Able to Hydrolyse R-Ketoprofen Amide

Volume: 15 Issue: 6

Author(s): Cesare Giordano and Sergio Ammendola

Affiliation:

Keywords: Enantioselectivity, Amidase, Mutation, Modelling, R-Ketoprofen

Abstract: The amidase from Sulfolobus solfataricus enantioselectively hydrolyzes S-ketoprofen amide to its corresponding acid. We identify three independent SsAH mutants that hydrolyze R-ketoprofen amide and built computational models of their three-dimensional structure. Interestingly the mutations do not specifically affect residues near the active site, or directly interacting with the substrate.

Cite this article as:

Giordano Cesare and Ammendola Sergio, Characterization of Mutants of Sulfolobus solfataricus Signature Amidase Able to Hydrolyse R-Ketoprofen Amide, Protein & Peptide Letters 2008; 15 (6) . https://dx.doi.org/10.2174/092986608784966949