The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro

Title: The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro

Volume: 8 Issue: 4

Author(s): Dong-Ning Wang, Lin Chen, Jie-Wu Liu, Zhi-Yong He, Wei-Jie Zhang and Xiang-Fu Wu

Affiliation:

Keywords: ANTI-LPS FACTOR, TACHYPLEUS TRIDENTATUS, CLONING, EXPRESSION, BACTERIOSTATIC ACTIVITY, Lipopolysaccharide (LPS), Endotoxin, Limulus polyphemus, Degenerate Oligonucleotides, LPS neutralization assay, Bacteriostatic assay

Abstract: The native gene information of anti-LPS factor from Tachypleus tridentatus was first reported in this study. The total RNA was extracted from amebocytes of Tachypleus tridentatus and the first-strand cDNA was synthesized by RT-PCR. Then the amplified cDNA of TALF was cloned and expressed in Pichia pastoris. The biological activity of the recombinant proteins obtained have been confirmed by LPS neutralization assay and bacteriostatic assay in vitro.

Cite this article as:

Wang Dong-Ning, Chen Lin, Liu Jie-Wu, He Zhi-Yong, Zhang Wei-Jie and Wu Xiang-Fu, The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro, Protein & Peptide Letters 2001; 8 (4) . https://dx.doi.org/10.2174/0929866013409337

DOI https://dx.doi.org/10.2174/0929866013409337	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305