One-Step Affinity Purification of Human Mu-Opioid Receptor Overexpressed in Baculovirus System

Title: One-Step Affinity Purification of Human Mu-Opioid Receptor Overexpressed in Baculovirus System

Volume: 8 Issue: 4

Author(s): Chen Li. Wei, Feng Ya. Ping, Zhou De He, Xu xue-Jun, Chen Jie, Wei Qiang and Chi Zhi-Qiang

Affiliation:

Keywords: BACULOVIRUS, opioid receptor (MOR), Spodoptera frugiperda, Trichoplusia ni, Receptor binding assay

Abstract: Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.

Cite this article as:

Wei Li. Chen, Ping Ya. Feng, He De Zhou, xue-Jun Xu, Jie Chen, Qiang Wei and Zhi-Qiang Chi, One-Step Affinity Purification of Human Mu-Opioid Receptor Overexpressed in Baculovirus System, Protein & Peptide Letters 2001; 8 (4) . https://dx.doi.org/10.2174/0929866013409373

DOI https://dx.doi.org/10.2174/0929866013409373	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305