Smartphone-Based Colourimetric Detection of Methyl Red, Co(II),
Uric Acid, and Topotecan after Pre-concentration onto a Hectorite
Clay-Hydroxyethylcellulose Hybrid

Anastasios Phoebus      Mazarakis; Georgia Eleni      Tsotsou
doi:10.2174/0115734110290080240314043658
Abstract

Objective: This paper describes a new, digital image colourimetry-based format for the quantification of analytes in an aqueous solution.
Methods: The proposed method is based on analyte pre-concentration by adsorption onto Bentone LT. Bentone LT pellet isolation comes after adsorption, followed by in-situ application of an analyteselective chromogenic reaction. The resulting pellet colouration is captured by the phone’s integrated camera and assessed using the free open-source image processing software, ImageJ. Responses are calibrated and quantified.
Results: We tested the applicability of the proposed methodology for the quantification of specific model analytes which are of concern in environmental matrices (methyl red, Co(II), uric acid, topotecan). The smartphone-based assay was proven reliable in quantifying the model analytes (standard recovery of 82-116%), alone or in mixture, from dilute aqueous solutions and was found to depict accurately the adsorption behaviour followed photometrically in solution. Lower limit of linearity was calculated at 0.05, 0.11, 0.85 and 0.20 μg/mL for methyl red, Co(II), uric acid, and topotecan, respectively. The proposed format was found superior when compared to alternative published photometric/ colourimetric assays in terms of the lower limit of linearity. In the presence of possible adsorption interferents, the lower limit of linear response was shifted to slightly higher concentrations for topotecan i.e. from 0.2 μg/mL to 0.5 μg/mL.
Conclusion: We here demonstrate the extended applicability of the proposed methodology for the smartphone-based quantification of the specific model analytes. The applicability of this analysis format likely extends to other analytes, where analyte-specific colour formation is feasible.
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Graphical Abstract

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DOI https://dx.doi.org/10.2174/0115734110290080240314043658	Print ISSN 1573-4110
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Current Analytical Chemistry

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