Abstract
In the absence of crystallographic data, NMR has emerged as the best way to define protein-ligand interactions. Using NMR experiments based on magnetization transfer, one can sort bound from unbound molecules, estimate the dissociation constant, identify contacts implied in the binding, characterize the structure of the bound ligand and conduct ligand competition assays.
Keywords: STD-NMR, WaterLOGSY, epitope mapping, docking, phosphorylated peptide, TrCP complex, binding fragment, protein-ligand interactions, magnetization transfer, dissociation constant, ligand competition assays, ATF4, TrCP, HSQC, MBP, NOESY, ROESY, SCF, TOCSY
Mini-Reviews in Medicinal Chemistry
Title: NMR Applications for Identifying β-TrCP Protein-Ligand Interactions
Volume: 11 Issue: 4
Author(s): J. Pons, V. Tanchou, J.-P. Girault, G. Bertho and N. Evrard-Todeschi
Affiliation:
Keywords: STD-NMR, WaterLOGSY, epitope mapping, docking, phosphorylated peptide, TrCP complex, binding fragment, protein-ligand interactions, magnetization transfer, dissociation constant, ligand competition assays, ATF4, TrCP, HSQC, MBP, NOESY, ROESY, SCF, TOCSY
Abstract: In the absence of crystallographic data, NMR has emerged as the best way to define protein-ligand interactions. Using NMR experiments based on magnetization transfer, one can sort bound from unbound molecules, estimate the dissociation constant, identify contacts implied in the binding, characterize the structure of the bound ligand and conduct ligand competition assays.
Export Options
About this article
Cite this article as:
Pons J., Tanchou V., Girault J.-P., Bertho G. and Evrard-Todeschi N., NMR Applications for Identifying β-TrCP Protein-Ligand Interactions, Mini-Reviews in Medicinal Chemistry 2011; 11 (4) . https://dx.doi.org/10.2174/138955711795305344
DOI https://dx.doi.org/10.2174/138955711795305344 |
Print ISSN 1389-5575 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5607 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Erythropoietin: New Approaches to Improved Molecular Designs and Therapeutic Alternatives
Current Pharmaceutical Design <i>In Silico</i> Study Examining New Phenylpropanoids Targets with Antidepressant Activity
Current Drug Targets Necroptosis: Who Knew There were so Many Interesting Ways to Die?
CNS & Neurological Disorders - Drug Targets Formononetin Ameliorates Cognitive Disorder via PGC-1α Pathway in Neuroinflammation Conditions in High-Fat Diet-Induced Mice
CNS & Neurological Disorders - Drug Targets Editorial [Hot Topic:Human Immunodeficiency Virus (HIV) and Hepatitis C Virus (HCV) (Guest Editor: Christopher J. Burns)]
Current Medicinal Chemistry - Anti-Infective Agents Challenges and Perspectives of Antiplatelet Therapy in Patients with Diabetes Mellitus and Coronary Artery Disease
Current Pharmaceutical Design Role of Defensins and Cathelicidin LL37 in Auto-Immune and Auto- Inflammatory Diseases
Current Pharmaceutical Biotechnology Identification of Candidate Genetic Markers and a Novel 4-genes Diagnostic Model in Osteoarthritis through Integrating Multiple Microarray Data
Combinatorial Chemistry & High Throughput Screening Validity of Oxygen-Ozone Therapy as Integrated Medication Form in Chronic Inflammatory Diseases
Cardiovascular & Hematological Disorders-Drug Targets Inducible Nitric Oxide Synthase as a Possible Target in Hypertension
Current Drug Targets Complications of Diabetes: An Insight into Genetic Polymorphism and Role of Insulin
Recent Patents on Inflammation & Allergy Drug Discovery Calcium Channels and Prostate Cancer
Recent Patents on Anti-Cancer Drug Discovery Basement Membrane Peptides: Functional Considerations and Biomedical Applications in Autoimmunity
Current Medicinal Chemistry Functional Roles of the Ca2+-activated K+ Channel, KCa3.1, in Brain Tumors
Current Neuropharmacology Growth Factors, Cytokines and Dendritic Cell Development
Current Pharmaceutical Design Tinea Capitis: An Updated Review
Recent Patents on Inflammation & Allergy Drug Discovery Allosteric Regulators of the Proteasome: Potential Drugs and a Novel Approach for Drug Design
Current Medicinal Chemistry Patent Selections
Recent Patents on Inflammation & Allergy Drug Discovery New Frontiers in Regenerative Medicine in Cardiology: The Potential of Wharton’s Jelly Mesenchymal Stem Cells
Current Stem Cell Research & Therapy Targeting Toll-like Receptors in Autoimmunity
Current Drug Targets