Profiling of Enzymatic Activities Using Peptide Arrays

Title: Profiling of Enzymatic Activities Using Peptide Arrays

Volume: 8 Issue: 2

Author(s): Alexandra Thiele, Stephanie Posel, Michael Spinka, Johannes Zerweck, Ulf Reimer, Ulrich Reineke and Mike Schutkowski

Affiliation:

Keywords: Kinase, peptide microarray, phosphatase, protease, SPOT synthesis, substrate specificity, enzyme profiling, ATP-ribosyltransferases

Abstract: Applications of peptide arrays in the field of enzyme profiling are described in detail. Focus is on assay principles for measuring activities of kinases, phosphatases or proteases and on substrate identification/optimization for kinases. Additionally, several examples for reliable identification of substrates for other enzymes like lysine methyl transferases and ATP-ribosyltransferases are given. Finally, use of high-density peptide microarrays for the simultaneous profiling of kinase activities in complex biological fluids like cell lysates or lysates of complete organisms will be analysed. All published examples of peptide arrays used for enzyme profiling are summarized comprehensively.

Cite this article as:

Thiele Alexandra, Posel Stephanie, Spinka Michael, Zerweck Johannes, Reimer Ulf, Reineke Ulrich and Schutkowski Mike, Profiling of Enzymatic Activities Using Peptide Arrays, Mini-Reviews in Organic Chemistry 2011; 8 (2) . https://dx.doi.org/10.2174/157019311795177772