Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase

Title: Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase

Volume: 17 Issue: 5

Author(s): Berivan Tandogan and N. Nuray Ulusu

Affiliation:

Keywords: Glutathione reductase, purification, kinetics, branched mechanism

Abstract: Glutathione reductase was purified 34806-fold with a final yield of 85 % from the bovine kidney cortex. Some molecular and kinetic properties of purified enzyme are investigated. Product inhibition studies showed that the enzyme obeys ‘branched’ mechanism: KmNADPH 18 ± 3 μM and KmGSSG 65 ± 5 μM were determined.

Cite this article as:

Tandogan Berivan and Ulusu N. Nuray, Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase, Protein & Peptide Letters 2010; 17 (5) . https://dx.doi.org/10.2174/092986610791112684

DOI https://dx.doi.org/10.2174/092986610791112684	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305