Stabilization of Folding Intermediate States from Alkaline Induced Unfolded State of Bovine Serum Fetuin in Trifluoroethanol and Acetonitrile

Title: Stabilization of Folding Intermediate States from Alkaline Induced Unfolded State of Bovine Serum Fetuin in Trifluoroethanol and Acetonitrile

Volume: 17 Issue: 5

Author(s): Basir Ahmad, Zeyaul Islam, Ankita Varshney and Rizwan Hasan Khan

Affiliation:

Keywords: Acetonitrile, alkali denaturation, bovine serum fetuin, circular dichroism, trifluoroethanol

Abstract: The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichroism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hydrophobic clusters.

Cite this article as:

Ahmad Basir, Islam Zeyaul, Varshney Ankita and Hasan Khan Rizwan, Stabilization of Folding Intermediate States from Alkaline Induced Unfolded State of Bovine Serum Fetuin in Trifluoroethanol and Acetonitrile, Protein & Peptide Letters 2010; 17 (5) . https://dx.doi.org/10.2174/092986610791112774