Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris

Title: Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris

Volume: 15 Issue: 4

Author(s): C. P.S. Carvalho, C. S. Rocha, D. R. Nepomuceno, J. T.A. Oliveira and T. B. Grangeiro

Affiliation:

Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast

Abstract: A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.

Cite this article as:

Carvalho P.S. C., Rocha S. C., Nepomuceno R. D., Oliveira T.A. J. and Grangeiro B. T., Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris, Protein & Peptide Letters 2008; 15 (4) . https://dx.doi.org/10.2174/092986608784246579