The Surface Plasmon Resonance Imaging Sensor for Papain Based on Immobilized Cystatin

Title: The Surface Plasmon Resonance Imaging Sensor for Papain Based on Immobilized Cystatin

Volume: 14 Issue: 5

Author(s): E. Gorodkiewicz

Affiliation:

Keywords: surface plasmon resonance imaging, papain, cystatin

Abstract: The Surface Plasmon Resonance Imaging (SPRI) sensor for papain determination based on the interaction between the immobilized cystatin and aqueous papain solution has been developed. The development of the sensor is a stage in the development of the sensor for the determination of cystein proteinases of the papain group. Cystatin was immobilized onto a gold chip by means of a thiol underlayer (cysteamine) and EDS/NHS reaction. Conditions of cystatin- papain interaction were optimized (pH, time of interaction and cystatin concentration). The developed sensor works within the range of 1 – 10 ng ml-1. However, the precision of measurements (RSD equal to 45%) should be improved. The response of the sensor to papain is specific. This was checked using BSA as a reference.

Cite this article as:

Gorodkiewicz E., The Surface Plasmon Resonance Imaging Sensor for Papain Based on Immobilized Cystatin, Protein & Peptide Letters 2007; 14 (5) . https://dx.doi.org/10.2174/092986607780782803

DOI https://dx.doi.org/10.2174/092986607780782803	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305