Comparative Antioxidant Level in Renal and Liver Tissues of Mice Treated with Fixed Dose Combination of Cefepime-Amikacin Reconstituted in Solvent vs Water for Injection

Arvind      Soni; Vivek   Kumar   Dwivedi; Kailash      Malik; Manu      Chaudhary

doi:10.2174/157488509789055018

Abstract

In order to investigate the protective role of cefepime plus amikacin (Potentox) reconstituted with solvent for injection vs cefepime and amikacin alone as well as potentox reconstituted with water for injection, the mice were fed standard pelleted diet and water ad libitum. The test room was air conditioned with temperature 23 ± 2oC, humidity 65± 5% and with artificial fluorescent light (10-14 hrs. of light and dark, respectively). Thirty Mus musculus mice (weighing 30 ± 5 g) were divided into five groups containing six mice in each group. Group- I Control (Normal Saline), Group-II cefepime (28.5 mg/Kg body weight/day), Group-III amikacin (7.14mg/Kg body weight/day), Group-IV cefepime plus amikacin reconstituted with water for injection (35.7mg/Kg body weight/day) and Group-V cefepime plus amikacin reconstituted with solvent for injection (35.7mg/Kg body weight/day). The level of antioxidant enzymes such as superoxide dismutase and catalase along with malonaldialdehyde (free radical mediated damage) and some extracellular levels namely creatinine, uric acid, serum glutamyl oxaloacetic transaminase and serum glutamyl pyruvic transaminase were also analyzed. In cefepime, amikacin and potentox reconstituted with water for injection treated groups, antioxidant enzyme activities were significantly decreased and the level of malonaldialdehyde as well as oxidant levels were significantly higher than those in the control group. Our results indicated that the protective effect of potentox reconstituted with solvent for injection increases the activity of antioxidant enzymes with the reduction of lipid peroxidation and oxidant level. These findings suggest that fixed dose combination of cefepime plus amikacin (Potentox) reconstituted with solvent for injection was found to be more effective and improve the activities of free radical scavenging enzymes.

Keywords: Fixed dose combination, cefepime, amikacin, free radical, Potentox, antioxidant enzymes, malonaldialdehyde

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