Effects of Plasticisers and Related Compounds on the Expression of the Soluble Form of Catechol-O-Methyltransferase in MCF-7 Cells

Title: Effects of Plasticisers and Related Compounds on the Expression of the Soluble Form of Catechol-O-Methyltransferase in MCF-7 Cells

Volume: 9 Issue: 4

Author(s): P. W.L. Ho, A. C.Y. Chu, K. H.H. Kwok, H. F. Liu, M. H.W. Kung, D. B. Ramsden and S. L. Ho

Affiliation:

Keywords: COMT, plasticiser, phthalate, endocrine disruption, estrogen

Abstract: Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.

Cite this article as:

Ho W.L. P., Chu C.Y. A., Kwok H.H. K., Liu F. H., Kung H.W. M., Ramsden B. D. and Ho L. S., Effects of Plasticisers and Related Compounds on the Expression of the Soluble Form of Catechol-O-Methyltransferase in MCF-7 Cells, Current Drug Metabolism 2008; 9 (4) . https://dx.doi.org/10.2174/138920008784220628