Abstract
Nuclear magnetic resonance (NMR) has become established as a powerful tool for qualitative and semi-quantitative analysis of complex mixtures of body fluids, intact cells, tissues and their extracts. Such studies are finding increasing applications in clinical and medical sciences. 1H and 31P NMR provide a noninvasive probe of high-energy compounds, amino acids, and compounds of phospholipid metabolism in cells and tissues. 13C NMR has been used to measure and characterize high concentration of molecules. The introduction of 13C-labeled substrates allows one to follow metabolism by the 13C NMR method. In this review, we have described one dimensional inverse detection techniques and various two dimensional homonuclear and heteronuclear methods with special emphasis on methods for studies of cells. Application of NMR to detect me tabolites in various cells and tissues has been discussed with illustrative examples, to highlight the type of information NMR can provide.
Current Organic Chemistry
Title: Application of NMR to the Study of Cells and Body Fluids
Volume: 5 Issue: 10
Author(s): Sudha Srivastava and Girjesh Govil
Affiliation:
Abstract: Nuclear magnetic resonance (NMR) has become established as a powerful tool for qualitative and semi-quantitative analysis of complex mixtures of body fluids, intact cells, tissues and their extracts. Such studies are finding increasing applications in clinical and medical sciences. 1H and 31P NMR provide a noninvasive probe of high-energy compounds, amino acids, and compounds of phospholipid metabolism in cells and tissues. 13C NMR has been used to measure and characterize high concentration of molecules. The introduction of 13C-labeled substrates allows one to follow metabolism by the 13C NMR method. In this review, we have described one dimensional inverse detection techniques and various two dimensional homonuclear and heteronuclear methods with special emphasis on methods for studies of cells. Application of NMR to detect me tabolites in various cells and tissues has been discussed with illustrative examples, to highlight the type of information NMR can provide.
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Sudha Srivastava and Girjesh Govil , Application of NMR to the Study of Cells and Body Fluids, Current Organic Chemistry 2001; 5 (10) . https://dx.doi.org/10.2174/1385272013374897
DOI https://dx.doi.org/10.2174/1385272013374897 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
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Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
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