Abstract
Nuclear magnetic resonance (NMR) has become established as a powerful tool for qualitative and semi-quantitative analysis of complex mixtures of body fluids, intact cells, tissues and their extracts. Such studies are finding increasing applications in clinical and medical sciences. 1H and 31P NMR provide a noninvasive probe of high-energy compounds, amino acids, and compounds of phospholipid metabolism in cells and tissues. 13C NMR has been used to measure and characterize high concentration of molecules. The introduction of 13C-labeled substrates allows one to follow metabolism by the 13C NMR method. In this review, we have described one dimensional inverse detection techniques and various two dimensional homonuclear and heteronuclear methods with special emphasis on methods for studies of cells. Application of NMR to detect me tabolites in various cells and tissues has been discussed with illustrative examples, to highlight the type of information NMR can provide.
Current Organic Chemistry
Title: Application of NMR to the Study of Cells and Body Fluids
Volume: 5 Issue: 10
Author(s): Sudha Srivastava and Girjesh Govil
Affiliation:
Abstract: Nuclear magnetic resonance (NMR) has become established as a powerful tool for qualitative and semi-quantitative analysis of complex mixtures of body fluids, intact cells, tissues and their extracts. Such studies are finding increasing applications in clinical and medical sciences. 1H and 31P NMR provide a noninvasive probe of high-energy compounds, amino acids, and compounds of phospholipid metabolism in cells and tissues. 13C NMR has been used to measure and characterize high concentration of molecules. The introduction of 13C-labeled substrates allows one to follow metabolism by the 13C NMR method. In this review, we have described one dimensional inverse detection techniques and various two dimensional homonuclear and heteronuclear methods with special emphasis on methods for studies of cells. Application of NMR to detect me tabolites in various cells and tissues has been discussed with illustrative examples, to highlight the type of information NMR can provide.
Export Options
About this article
Cite this article as:
Sudha Srivastava and Girjesh Govil , Application of NMR to the Study of Cells and Body Fluids, Current Organic Chemistry 2001; 5 (10) . https://dx.doi.org/10.2174/1385272013374897
DOI https://dx.doi.org/10.2174/1385272013374897 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
Call for Papers in Thematic Issues
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
The Potential for Substance P Antagonists as Anti-Cancer Agents in Brain Tumours
Recent Patents on CNS Drug Discovery (Discontinued) Tracking Stem Cells for Cellular Therapy in Stroke
Current Pharmaceutical Design Recent Patents Concerning Modulators of Protein Kinase C
Recent Patents on DNA & Gene Sequences Biological Relevance of DNA Polymerase Beta and Translesion Synthesis Polymerases to Cancer and its Treatment
Current Molecular Pharmacology Pharmacological Modulation of Sphingolipids and Role in Disease and Cancer Cell Biology
Mini-Reviews in Medicinal Chemistry Tumor Targeting with RGD Peptide Ligands-Design of New Molecular Conjugates for Imaging and Therapy of Cancers
Anti-Cancer Agents in Medicinal Chemistry The Molecular Mechanisms of TRAIL Resistance in Cancer Cells: Help in Designing New Drugs
Current Pharmaceutical Design Recent Progress in Anticancer Agents Incorporating Pyrazole Scaffold
Mini-Reviews in Medicinal Chemistry Active Tumor Targeting of Nanomaterials Using Folic Acid, Transferrin and Integrin Receptors
Current Drug Discovery Technologies Nanoparticles: Properties and Applications in Cancer Immunotherapy
Current Pharmaceutical Design Functions of S100 Proteins
Current Molecular Medicine Multidrug-Resistance (MDR) Proteins Develops Refractory Epilepsy Phenotype:Clinical and Experimental Evidences
Current Drug Therapy Peptide-based Radiopharmaceuticals for Targeted Tumor Therapy
Current Medicinal Chemistry Magnetic Nanoparticles in Brain Disease Diagnosis and Targeting Drug Delivery
Current Nanoscience Targeting JAK/STAT Signaling Pathway in Inflammatory Diseases
Current Signal Transduction Therapy Immunologic Diseases and Brain Tumors
Inflammation & Allergy - Drug Targets (Discontinued) Harnessing the Capacity of Cell-Penetrating Peptides for Drug Delivery to the Central Nervous System
Current Pharmaceutical Biotechnology MicroRNAs: Key Players in Microglia and Astrocyte Mediated Inflammation in CNS Pathologies
Current Medicinal Chemistry Mechanisms and Inhibitors of Apoptosis in Cardiovascular Diseases
Current Pharmaceutical Design Impact of Cellular Senescence in Aging and Cancer
Current Pharmaceutical Design