Generic placeholder image

Current Pharmaceutical Design

Editor-in-Chief

ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

Activated Surfaces for Laser Desorption Mass Spectrometry: Application for Peptide and Protein Analysis

Author(s): C. Afonso, N. Budimir, F. Fournier and J- C. Tabet

Volume 11, Issue 20, 2005

Page: [2559 - 2576] Pages: 18

DOI: 10.2174/1381612054546888

Price: $65

conference banner
Abstract

Thanks to the development of matrix assisted laser desorption/ionisation (MALDI), laser desorption based mass spectrometry became an essential method for the analysis of biomolecules. This review will discuss the various surface modifications used in combination with laser desorption mass spectrometry and their application for the analysis of peptides and proteins. In the first hand, some modified surfaces are designed to enhance the laser desorption/ionisation process; this includes the use of carbon, porous silicon surfaces and also immobilised matrix. In an other hand chemical and biochemical modified surfaces developed to isolate species with more or less specific interactions can be used for onslide sample clean-up before MALDI-MS analysis. In addition, different experimental devices as mass spectrometers and microfluidic devices used for such a purpose will be presented.

Keywords: field desorption (fd), laser microprobe, chromatography, porous silicon, photo-responsive monomers, microarrays, self-assembled monolayer (sams), microfluidic devices


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy