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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

Antimicrobial and Antifungal Properties of Leaves to Root Extracts and Saponin Fractions of Chlorophytum borivilianum

Author(s): Pallavi Sharma, Vineeta Singh, Sanjeev K. Maurya, Mohammad A. Kamal and Nitesh K. Poddar*

Volume 17, Issue 6, 2021

Published on: 06 October, 2020

Article ID: e010621186650 Pages: 10

DOI: 10.2174/1573407216999201006124428

Price: $65

Abstract

Objective: The study was conducted to examine the antimicrobial activity of methanolic crude extract from different parts of the Chlorophytum borivilianum plant against pathogenic microorganisms and toassess antimicrobial activity by MIC and structural characterization of purified saponin of Chlorophytum borivilianum by using spectrophotometric and NMR analysis.

Methods: The antimicrobial analysis of the extracts of leaves, roots and stems of C. borivilianum is based on the agar well diffusion method and Minimum Inhibitory Concentration (MIC). The phytochemical screening and characterization of saponin on the basis of structural and antimicrobial activity present in C. borivilianum were analyzed by different spectrophotometric methods such as HPLC, UV-visible, IR, NMR, LC-ESI-MS and pharmacophore modeling.

Results: The results revealed that the methanolic leaf, stem and root extracts have inhibitory potential against the growth of K. pneumonia, B. subtilis, M. tuberculosis, E coli and S. aureus in case of bacteria and C. albicans, A. fumigatus and Tricoderma in case of fungus. The MIC values of leaf, stem and root extracts were found in the range of 1 mg/ml to 0.125 mg/ml. Moreover, the purified saponins indicated MIC in the range of 0.5 mg/ml to 0.0625 mg/ml against the selected microbial pathogens. Saponins act as one of the major phytocomponents present in C. borivilianum. The antimicrobial and structural analysis of purified saponins of C. borivilianum was also performed using different spectral analysis methods.

Conclusion: The anti-microbial results showed that the extract from the leaf and stems had higher anti-pathogenic activity as compared to the roots. The MIC results showed that the purified saponin also possessed the anti-microbial activity and oleanolic acid content, as detected by spectral analysis the fundamental structure of the extracted saponin.

Keywords: Phytochemicals, saponin, anti-microbial, anti-fungal, medicinal, anti-bacterial, C. borivilianum.

Graphical Abstract

[1]
Medzhitov R. Recognition of microorganisms and activation of the immune response. Nature 2007; 449(7164): 819-26.
[http://dx.doi.org/10.1038/nature06246] [PMID: 17943118]
[2]
Khan AU, Zaman MS. Multiple drug resistance pattern in Urinary Tract Infection (UTI) patients in Aligarh. Biomed Res 2006; 17(3): 179-81.
[3]
Cohen T, van Helden PD, Wilson D, et al. Mixed-strain mycobacterium tuberculosis infections and the implications for tuberculosis treatment and control. Clin Microbiol Rev 2012; 25(4): 708-19.
[http://dx.doi.org/10.1128/CMR.00021-12] [PMID: 23034327]
[4]
D’Costa VM, King CE, Kalan L, et al. Antibiotic resistance is ancient. Nature 2011; 477(7365): 457-61.
[http://dx.doi.org/10.1038/nature10388] [PMID: 21881561]
[5]
Levy SB, Marshall B. Antibacterial resistance worldwide: causes, challenges and responses. Nat Med 2004; 10(12)(Suppl.): S122-9.
[http://dx.doi.org/10.1038/nm1145] [PMID: 15577930]
[6]
Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med 2011; 8(1): 1-10.
[PMID: 22238476]
[7]
Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complement Altern Med 2006; 6(1): 35-41.
[http://dx.doi.org/10.1186/1472-6882-6-35] [PMID: 17042964]
[8]
Elaloui M, Ennajah A, Ghazghazi H, et al. A comparative phytochemical and biological study between different solvent extracts of leaves and stems extracts of Erica arborea L. and Viburnum tinus L. Plants Growing in Tunisia. Curr Bioact Compd 2019; 15: 686.
[http://dx.doi.org/10.2174/1573407214666180730110232]
[9]
Golkar P, Fotoohi A, Frezza C. Preliminary phytochemical screening, evaluation of the phenolic compositions and antioxidant activities of four Iranian alyssum species. Curr Bioact Compd 2020; 16(5): 581-7.
[http://dx.doi.org/10.2174/1573407215666190215152137]
[10]
Das A, Shakya A, Ghosh SK, Singh UP, Bhat HR. A review of phytochemical and pharmacological studies of inula species. Curr Bioact Compd 2020; 16(5): 557-67.
[http://dx.doi.org/10.2174/1573407215666190207093538]
[11]
Bathoju G, Rao K, Giri A. Production of sapogenins (stigmasterol and hecogenin) from genetically transformed hairy root cultures of Chlorophytum borivilianum (Safedmusli). Plant Cell Tissue Organ Cult 2017; 131(3): 369-76.
[http://dx.doi.org/10.1007/s11240-017-1290-8]
[12]
Bee LC, Zunoliza A, Kar YP, Luqman CA, Thomas SYC, Umi KY. Isolation, structure elucidation, identification and quantitative analysis of 1′-acetoxychavicol (ACA) from the roots of Chlorophytum boriviliuanum (safedmusli). J Eng Sci Technol 2017; 12(1): 198-213.
[13]
Khanam Z, Singh O, Singh R, Bhat IUH. Safed musli (Chlorophytum borivilianum): A review of its botany, ethnopharmacology and phytochemistry. J Ethnopharmacol 2013; 150(2): 421-41.
[http://dx.doi.org/10.1016/j.jep.2013.08.064] [PMID: 24045177]
[14]
Verma R, Misra V, Bisen PS. Nutritional and medicinal values of Chlorophytum borivilianum: Mini-review of current status and future possibilities. Curr Nutr Food Sci 2020; 16: 1-8.
[http://dx.doi.org/10.2174/1573401316666200225122210]
[15]
Haque R, Saha S, Bera T. A Peer Reviewed of General Literature on Chlorophytum borivilianum Commercial Medicinal Plant. Int J Drug Dev Res 2011; 3(1): 140-55.
[16]
Kumar D, Bhatnagar SP. Pharmacognostical evaluation of chlorophytum borivilianum root. Anc Sci Life 2004; 24(1): 30-7.
[PMID: 22557148]
[17]
Acharya D, Mitaine-Offer AC, Kaushik N, et al. Cytotoxic spirostane-type saponins from the roots of Chlorophytum borivilianum. J Nat Prod 2009; 72(1): 177-81.
[http://dx.doi.org/10.1021/np800559z] [PMID: 19128156]
[18]
Ved D, Saha D, Ravikumar K, Haridasan K. Chlorophytum borivilianum. IUCN Red List Threat Species 2007; 8235: 2007.
[19]
Ahmad SR, Kalam A, Pal K. Phytochemical Analysis and antimicrobial activity of Chlorophytum borivilianum against bacterial pathogen causing disease in Humans. Int J Appl Sci Eng 2014; 2(2): 83-9.
[http://dx.doi.org/10.5958/2322-0465.2015.00001.5]
[20]
Ghorpade DS, Thakare PV. Phytochemical screening and antimicrobial activity of Chlorophytum species leaves of Melghat region. Int J Pharmacogn Phytochem Res 2014; 6(1): 141-5.
[21]
Deore SL, Khadabadi SS. Standardization and pharmaceutical evaluation of Chlorophytum borivilianum mucilage. Rasayan J Chem 2008; 1(4): 887-92.
[22]
Luangtongkum T, Morishita TY, El-Tayeb AB, Ison AJ, Zhang Q. Comparison of antimicrobial susceptibility testing of Campylobacter spp. by the agar dilution and the agar disk diffusion methods. J Clin Microbiol 2007; 45(2): 590-4.
[http://dx.doi.org/10.1128/JCM.00986-06] [PMID: 17122005]
[23]
Rondevaldova J, Novy P, Urban J, Kokoska L. Determination of anti-staphylococcal activity of thymoquinone in combinations with antibiotics by checkerboard method using EVA capmat™ as a vapor barrier. Arab J Chem 2017; 10(4): 566-72.
[http://dx.doi.org/10.1016/j.arabjc.2015.04.021]
[24]
Elmastaş M, Gülçin I, Işildak Ö, Küfrevioğlu Öİ, İbaoğlu K, Aboul-Enein HY. Radical scavenging activity and antioxidant capacity of bay leaf extracts. J Indian Chem Soc 2006; 3(3): 258-66.
[http://dx.doi.org/10.1007/BF03247217]
[25]
Gülçin I. The antioxidant and radical scavenging activities of black pepper (Piper nigrum) seeds. Int J Food Sci Nutr 2005; 56(7): 491-9.
[http://dx.doi.org/10.1080/09637480500450248] [PMID: 16503560]
[26]
Satyanarayana V, Kumari SJ. Preliminary phytochemical screening and TLC profile of selected four plants of Tirupati hills in chitoor district, Andhra Pradesh. J Pharmacogn Phytochem 2016; 5(2): 259-64.
[27]
Athiralakshmy TR, Divyamol AS, Nisha P. Phytochemical screening of Saracaasoca and antimicrobial activities against bacterial species. Asian J Plant Sci 2016; 6(2): 30-6.
[28]
Sharma R, Saxena N, Thakur GS, Sanodiya BS, Jaiswal P. Conventional method for saponin extraction from Chlorophytum borivilianum Sant. Et Fernand. Glob J Res Med Plants Indig Med 2014; 3(2): 33-9.
[29]
Bhat MH, Fayaz M, Kumar A, Jain AK. Phytochemical, Pharmacological and Nutritional Profile of Chlorophytum tuberosum (Roxb.) Baker (Safedmusli): A Review. International Journal of Theoretical & Applied Sciences 2018; 10(1): 93-9.
[30]
Jones PW, Kinghorn AD. Extraction of plant secondary metabolites. Nat Prod Rep 2006; 20(2): 323-51.
[http://dx.doi.org/10.1385/1-59259-955-9:323]
[31]
Hussein RA, El-Anssary AA. Plants secondary metabolites: The key drivers of the pharmacological actions of medicinal plants.Herbal Medicine. Intech Open 2018.
[32]
Chakraborthy GS, Aeri V. Phytochemical and antimicrobial studies of Chlorophytum borivilianum. Int J Pharm Sci Drug Res 2009; 1(2): 110-2.
[33]
Lee EB, Kim OJ, Kang SS, Jeong C. Araloside A, an antiulcer constituent from the root bark of Aralia elata. Biol Pharm Bull 2005; 28(3): 523-6.
[http://dx.doi.org/10.1248/bpb.28.523] [PMID: 15744082]
[34]
Szakiel A, Ruszkowski D, Janiszowska W. Saponins in Calendula officinalis L–structure, biosynthesis, transport and biological activity. Phytochem Rev 2005; 4(2-3): 151-8.
[http://dx.doi.org/10.1007/s11101-005-4053-9]
[35]
Khathi A, Serumula MR, Myburg RB, Van Heerden FR, Musabayane CT. Effects of Syzygium aromaticum-derived triterpenes on postprandial blood glucose in streptozotocin-induced diabetic rats following carbohydrate challenge. PLoS One 2013; 8(11): e81632.
[http://dx.doi.org/10.1371/journal.pone.0081632] [PMID: 24278452]
[36]
Sundaram S, Dwivedi P, Purwar S. Antibacterial activities of crude extracts of Chlorophytum borivilianum to bacterial pathogens. Res J Med Plant 2011; 5(3): 343-7.
[http://dx.doi.org/10.3923/rjmp.2011.343.347]
[37]
Peleg AY, Hooper DC. Hospital-acquired infections due to gram-negative bacteria. N Engl J Med 2010; 362(19): 1804-13.
[http://dx.doi.org/10.1056/NEJMra0904124] [PMID: 20463340]
[38]
Singh RP, Desouky SE, Nakayama J. Quorum quenching strategy targeting Gram-positive pathogenic bacteria.Advances in Microbiology, Infectious Diseases and Public Health. Cham: Springer 2016; pp. 109-30.
[http://dx.doi.org/10.1007/5584_2016_1]
[39]
Ashraf MF, Abd Aziz M, Stanslas J, Ismail I, Abdul Kadir M. Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum borivilianum. ScientificWorldJournal 2013; 2013: 216894.
[http://dx.doi.org/10.1155/2013/216894] [PMID: 24223502]
[40]
Gülçin I, Mshvildadze V, Gepdiremen A, Elias R. Antioxidant activity of saponins isolated from ivy: Alpha-hederin, hederasaponin-C, hederacolchiside-E and hederacolchiside-F. Planta Med 2004; 70(6): 561-3.
[http://dx.doi.org/10.1055/s-2004-827158] [PMID: 15241892]
[41]
Visavadiya NP, Soni B, Dalwadi N, Madamwar D. Chlorophytum borivilianum as potential terminator of free radicals in various in vitro oxidation systems. Drug Chem Toxicol 2010; 33(2): 173-82.
[http://dx.doi.org/10.3109/01480540903311068] [PMID: 20307144]

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