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Protein & Peptide Letters

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Research Article

Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields

Author(s): Farzaneh Sadeghzadeh, Amir Arsalan Entezari, Kiana Behzadian, Kimia Habibi, Zeinab Amiri-Tehranizadeh*, Ahmad Asoodeh, Mohammad Reza Saberi and Jamshidkhan Chamani*

Volume 27, Issue 10, 2020

Page: [1007 - 1021] Pages: 15

DOI: 10.2174/1871530320666200425203636

Price: $65

Abstract

Background: Drug-protein complexes is one of the crucial factors when analyzing the pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution, metabolism and interaction with target tissues.

Objectives: The aim of this study was to investigate the interaction of human hemoglobin (Hb) and angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different- frequency electromagnetic fields (EMF).

Methods: Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the absence and presence of EMF.

Result: The presented spectroscopic studies indicated that EMF changed the interaction between Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the Hb-ACEIP affinity was higher in the presence of EMF.

Conclusion: It can be concluded that for patients who use the drug to control blood pressure, a low-frequency electromagnetic field would have a positive effect on the uptake of the drug.

Keywords: Drug-protein complexes, ACE inhibitory peptide, human hemoglobin, electromagnetic field, fluorescence spectroscopy, circular dichroism.

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