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Current Nutrition & Food Science

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ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

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Research Article

Sorghum (Sorghum bicolor) Extract Affects Plasma Lipid Metabolism and Hepatic Macrophage Infiltration in Diabetic Rats

Author(s): Yuuka Mukai*, Saori Kataoka and Shin Sato

Volume 16, Issue 5, 2020

Page: [824 - 832] Pages: 9

DOI: 10.2174/1573401315666190114153933

Abstract

Background: Chronic hyperglycemia is known to be a high-risk factor for progressive chronic liver diseases, such as abnormal lipid metabolism. The activation of AMP-activated protein kinase (AMPK) has a beneficial effect on dyslipidemia. Polyphenols derived from various plants are involved in AMPK activation.

Objective: We investigated the effects of polyphenol-containing sorghum (Sorghum bicolor) extract (SE) on plasma lipid metabolism and macrophage infiltration, and measured the expression and phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in diabetic rat livers.

Methods: Streptozotocin-induced diabetic rats received 0, 50, or 250 mg/kg of SE orally for 4 weeks. Blood chemistry, total and phosphorylated protein levels of AMPK and ACC, sterol regulatory element- binding protein-1c (SREBP-1c) mRNA and protein levels, and macrophage infiltration in the livers were examined.

Results: Plasma glucose and triacylglycerol levels, which were increased in the untreated diabetic rats, were significantly lower in the 250 mg/kg SE-treated diabetic rats. AMPK and ACC phosphorylation levels were significantly increased in the 250 mg/kg SE-treated diabetic rats compared with those in the untreated rats. There was no difference in the hepatic expression of SREBP-1c between the diabetic rat groups. Macrophage infiltration in the liver was suppressed by 250 mg/kg of SEtreatment.

Conclusion: These data suggest that SE treatment may affect plasma lipid metabolism and chronic inflammation by upregulating phosphorylation of AMPK and ACC in diabetic rat livers.

Keywords: Acetyl-CoA carboxylase, AMP-activated protein kinase, lipid metabolism, liver, macrophage infiltration, Sorghum bicolor, streptozotocin-induced diabetic rats.

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[1]
Sánchez-Zamora YI, Rodriguez-Sosa M. The role of MIF in type 1 and type 2 diabetes mellitus. J Diabetes Res 2014; 2014, 804519
[http://dx.doi.org/10.1155/2014/804519] [PMID: 24527464]
[2]
Arrese M. Nonalcoholic fatty liver disease: liver disease: an overlooked complication of diabetes mellitus. Nat Rev Endocrinol 2010; 6(12): 660-1.
[http://dx.doi.org/10.1038/nrendo.2010.173] [PMID: 21102646]
[3]
Erion DM, Park HJ, Lee HY. The role of lipids in the pathogenesis and treatment of type 2 diabetes and associated co-morbidities. BMB Rep 2016; 49(3): 139-48.
[http://dx.doi.org/10.5483/BMBRep.2016.49.3.268] [PMID: 26728273]
[4]
Xue B, Kahn BB. AMPK integrates nutrient and hormonal signals to regulate food intake and energy balance through effects in the hypothalamus and peripheral tissues. J Physiol 2006; 574(Pt 1): 73-83.
[http://dx.doi.org/10.1113/jphysiol.2006.113217] [PMID: 16709629]
[5]
Sanders FW, Griffin JL. De novo lipogenesis in the liver in health and disease: more than just a shunting yard for glucose. Biol Rev Camb Philos Soc 2016; 91(2): 452-68.
[http://dx.doi.org/10.1111/brv.12178] [PMID: 25740151]
[6]
Munday MR. Regulation of mammalian acetyl-CoA carboxylase. Biochem Soc Trans 2002; 30(Pt 6): 1059-64.
[http://dx.doi.org/10.1042/bst0301059] [PMID: 12440972]
[7]
Shaw RJ, Kosmatka M, Bardeesy N, et al. The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress. Proc Natl Acad Sci USA 2004; 101(10): 3329-35.
[http://dx.doi.org/10.1073/pnas.0308061100] [PMID: 14985505]
[8]
Ben Djoudi Ouadda A, Levy E, Ziv E, et al. Increased hepatic lipogenesis in insulin resistance and Type 2 diabetes is associated with AMPK signalling pathway up-regulation in Psammomys obesus. Biosci Rep 2009; 29(5): 283-92.
[http://dx.doi.org/10.1042/BSR20080141] [PMID: 18842111]
[9]
Foretz M, Pacot C, Dugail I, et al. ADD1/SREBP-1c is required in the activation of hepatic lipogenic gene expression by glucose. Mol Cell Biol 1999; 19(5): 3760-8.
[http://dx.doi.org/10.1128/MCB.19.5.3760] [PMID: 10207099]
[10]
Linden AG, Li S, Choi HY, et al. Interplay between ChREBP and SREBP-1c coordinates postprandial glycolysis and lipogenesis in livers of mice. J Lipid Res 2018; 59(3): 475-87.
[http://dx.doi.org/10.1194/jlr.M081836] [PMID: 29335275]
[11]
Yang Z, Kahn BB, Shi H, Xue BZ. Macrophage alpha1 AMP-activated protein kinase (alpha1AMPK) antagonizes fatty acid-induced inflammation through SIRT1. J Biol Chem 2010; 285(25): 19051-9.
[http://dx.doi.org/10.1074/jbc.M110.123620] [PMID: 20421294]
[12]
Ko HJ, Zhang Z, Jung DY, et al. Nutrient stress activates inflammation and reduces glucose metabolism by suppressing AMP-activated protein kinase in the heart. Diabetes 2009; 58(11): 2536-46.
[http://dx.doi.org/10.2337/db08-1361] [PMID: 19690060]
[13]
An H, He L. Current understanding of metformin effect on the control of hyperglycemia in diabetes. J Endocrinol 2016; 228(3): R97-R106.
[http://dx.doi.org/10.1530/JOE-15-0447] [PMID: 26743209]
[14]
Grahame Hardie D. Regulation of AMP-activated protein kinase by natural and synthetic activators. Acta Pharm Sin B 2016; 6(1): 1-19.
[http://dx.doi.org/10.1016/j.apsb.2015.06.002] [PMID: 26904394]
[15]
Do GM, Jung UJ, Park HJ, et al. Resveratrol ameliorates diabetes-related metabolic changes via activation of AMP-activated protein kinase and its downstream targets in db/db mice. Mol Nutr Food Res 2012; 56(8): 1282-91.
[http://dx.doi.org/10.1002/mnfr.201200067] [PMID: 22715031]
[16]
Zou B, Ge ZZ, Zhang Y, Du J, Xu Z, Li CM. Persimmon tannin accounts for hypolipidemic effects of persimmon through activating of AMPK and suppressing NF-κB activation and inflammatory responses in high-fat diet rats. Food Funct 2014; 5(7): 1536-46.
[http://dx.doi.org/10.1039/C3FO60635J] [PMID: 24841999]
[17]
Sato S, Mukai Y, Kataoka S, Kurasaki M. Azuki bean (Vigna angularis) extract stimulates the phosphorylation of AMP-activated protein kinase in HepG2 cells and diabetic rat liver. J Sci Food Agric 2016; 96(7): 2312-8.
[http://dx.doi.org/10.1002/jsfa.7346] [PMID: 26198388]
[18]
Afify Ael-M, El-Beltagi HS, El-Salam SM, Omran AA. Biochemical changes in phenols, flavonoids, tannins, vitamin E, β-carotene and antioxidant activity during soaking of three white sorghum varieties. Asian Pac J Trop Biomed 2012; 2(3): 203-9.
[http://dx.doi.org/10.1016/S2221-1691(12)60042-2] [PMID: 23569898]
[19]
Kim J, Park Y. Anti-diabetic effect of sorghum extract on hepatic gluconeogenesis of streptozotocin-induced diabetic rats. Nutr Metab (Lond) 2012; 9(1): 106.
[http://dx.doi.org/10.1186/1743-7075-9-106] [PMID: 23186010]
[20]
Chung IM, Kim EH, Yeo MA, et al. Antidiabetic effects of three Korean sorghum phenolic extracts in normal and streptozotocin-induced diabetic rats. Food Res Int 2011; 44(1): 127-32.
[http://dx.doi.org/10.1016/j.foodres.2010.10.051]
[21]
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965; 16: 144-58.
[22]
Steinberg GR, Kemp BE. AMPK in Health and Disease. Physiol Rev 2009; 89(3): 1025-78.
[http://dx.doi.org/10.1152/physrev.00011.2008] [PMID: 19584320]
[23]
Kanikarla-Marie P, Jain SK. Role of hyperketonemia in inducing oxidative stress and cellular damage in cultured hepatocytes and type 1 diabetic rat liver. Cell Physiol Biochem 2015; 37(6): 2160-70.
[http://dx.doi.org/10.1159/000438573] [PMID: 26606728]
[24]
Soetikno V, Sari FR, Sukumaran V, et al. Curcumin decreases renal triglyceride accumulation through AMPK-SREBP signaling pathway in streptozotocin-induced type 1 diabetic rats. J Nutr Biochem 2013; 24(5): 796-802.
[http://dx.doi.org/10.1016/j.jnutbio.2012.04.013] [PMID: 22898567]
[25]
Benhaddou-Andaloussi A, Martineau L, Vuong T, et al. The in vivo antidiabetic activity of Nigella sativa is mediated through activation of the AMPK pathway and increased muscle Glut4 content. Evid Based Complement Alternat Med 2011; 2011, 538671
[http://dx.doi.org/10.1155/2011/538671] [PMID: 21584245]
[26]
Dong J, Xu H, Xu H, et al. Nesfatin-1 stimulates fatty-acid oxidation by activating AMP-activated protein kinase in STZ-induced type 2 diabetic mice. PLoS One 2013; 8(12) e83397
[http://dx.doi.org/10.1371/journal.pone.0083397] [PMID: 24391760]
[27]
Kurimoto Y, Shibayama Y, Inoue S, et al. Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice. J Agric Food Chem 2013; 61(23): 5558-64.
[http://dx.doi.org/10.1021/jf401190y] [PMID: 23683106]
[28]
Awika JM, Rooney LW. Sorghum phytochemicals and their potential impact on human health. Phytochemistry 2004; 65(9): 1199-221.
[http://dx.doi.org/10.1016/j.phytochem.2004.04.001] [PMID: 15184005]
[29]
Zhang BB, Zhou G, Li C. AMPK: an emerging drug target for diabetes and the metabolic syndrome. Cell Metab 2009; 9(5): 407-16.
[http://dx.doi.org/10.1016/j.cmet.2009.03.012] [PMID: 19416711]
[30]
Viollet B, Guigas B, Leclerc J, et al. AMP-activated protein kinase in the regulation of hepatic energy metabolism: from physiology to therapeutic perspectives. Acta Physiol (Oxf) 2009; 196(1): 81-98.
[http://dx.doi.org/10.1111/j.1748-1716.2009.01970.x] [PMID: 19245656]
[31]
Zang M, Xu S, Maitland-Toolan KA, et al. Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes 2006; 55(8): 2180-91.
[http://dx.doi.org/10.2337/db05-1188] [PMID: 16873680]
[32]
Hou X, Xu S, Maitland-Toolan KA, et al. SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. J Biol Chem 2008; 283(29): 20015-26.
[http://dx.doi.org/10.1074/jbc.M802187200] [PMID: 18482975]
[33]
Santamarina AB, Oliveira JL, Silva FP, et al. Green tea extract rich in epigallocatechin-3-gallate prevents fatty liver by AMPK activation via LKB1 in mice fed a high-fat diet. PLoS One 2015; 10(11) e0141227
[http://dx.doi.org/10.1371/journal.pone.0141227] [PMID: 26536464]
[34]
Salt IP, Palmer TM. Exploiting the anti-inflammatory effects of AMP-activated protein kinase activation. Expert Opin Investig Drugs 2012; 21(8): 1155-67.
[http://dx.doi.org/10.1517/13543784.2012.696609] [PMID: 22694351]
[35]
Mancini SJ, White AD, Bijland S, et al. Activation of AMP-activated protein kinase rapidly suppresses multiple pro-inflammatory pathways in adipocytes including IL-1 receptor-associated kinase-4 phosphorylation. Mol Cell Endocrinol 2017; 440: 44-56.
[http://dx.doi.org/10.1016/j.mce.2016.11.010] [PMID: 27840174]
[36]
Olivier S, Foretz M, Viollet B. Promise and challenges for direct small molecule AMPK activators. Biochem Pharmacol 2018; 153: 147-58.
[http://dx.doi.org/10.1016/j.bcp.2018.01.049] [PMID: 29408352]
[37]
Kang JH, Tsuyoshi G, Le Ngoc H, et al. Dietary capsaicin attenuates metabolic dysregulation in genetically obese diabetic mice. J Med Food 2011; 14(3): 310-5.
[http://dx.doi.org/10.1089/jmf.2010.1367] [PMID: 21332406]
[38]
Cao Y, Bao S, Yang W, et al. Epigallocatechin gallate prevents inflammation by reducing macrophage infiltration and inhibiting tumor necrosis factor-α signaling in the pancreas of rats on a high-fat diet. Nutr Res 2014; 34(12): 1066-74.
[http://dx.doi.org/10.1016/j.nutres.2014.10.004] [PMID: 25453543]
[39]
Krishnan TR, Velusamy P, Srinivasan A, et al. EGCG mediated downregulation of NF-AT and macrophage infiltration in experimental hepatic steatosis. Exp Gerontol 2014; 57: 96-103.
[http://dx.doi.org/10.1016/j.exger.2014.05.008] [PMID: 24844145]
[40]
Matsumoto E, Kataoka S, Mukai Y, Sato M, Sato S. Green tea extract intake during lactation modified cardiac macrophage infiltration and AMP-activated protein kinase phosphorylation in weanling rats from undernourished mother during gestation and lactation. J Dev Orig Health Dis 2017; 8(2): 178-87.
[http://dx.doi.org/10.1017/S2040174416000647] [PMID: 27919304]
[41]
Stephenne X, Foretz M, Taleux N, et al. Metformin activates AMP-activated protein kinase in primary human hepatocytes by decreasing cellular energy status. Diabetologia 2011; 54(12): 3101-10.
[http://dx.doi.org/10.1007/s00125-011-2311-5] [PMID: 21947382]
[42]
Viollet B, Mounier R, Leclerc J, Yazigi A, Foretz M, Andreelli F. Targeting AMP-activated protein kinase as a novel therapeutic approach for the treatment of metabolic disorders. Diabetes Metab 2007; 33(6): 395-402.
[http://dx.doi.org/10.1016/j.diabet.2007.10.004] [PMID: 17997341]

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