In Vitro Effects of Nickel on Healthy Non-Allergic Peripheral Blood Mononuclear Cells. The Role of Red Grape Polyphenols

Title:In Vitro Effects of Nickel on Healthy Non-Allergic Peripheral Blood Mononuclear Cells. The Role of Red Grape Polyphenols

Volume: 17 Issue: 2

Author(s): Thea Magrone*, Rosaria Salvatore, Anna Spagnoletta, Manrico Magrone, Matteo Antonio Russo and Emilio Jirillo

Affiliation:

• Department of Basic Medical Sciences, Neuroscience and Sensory Organs, University of Bari, P.zza Giulio Cesare 11, 70124 Bari,Italy

Keywords: Lipopolysaccharides, nitric oxide, non-allergic, nickel individuals, peripheral blood mononuclear cells, polyphenols.

Abstract: Background: Nickel (Ni) is widely distributed in the environment and continuous exposure to this metal may lead to pathological manifestations, such as the human allergic contact dermatitis.

Methods: The in vitro effects of Ni on human healthy non allergic peripheral blood mononuclear cells (PBMCs) in the absence or presence of red grape polyphenols have been evaluated. In the culture supernatants, levels of cytokines have been determined by ELISA, while nitric oxide (NO) concentration has been evaluated by a colorimetric procedure.

Results: Ni per se did not affect release of interferon-γ, interleukin (IL)-4 and IL-10. Instead, this metal dramatically reduced production of IL-17 which was restored by the supplementation of polyphenols. Finally, while Ni significantly increased generation of NO, polyphenols reduced production of this compound.

Conclusion: Taken together, all these data may indicate a preventative role of polyphenols against Ni exposure in non allergic to Ni individuals, also confirming the immunomodulating role of these natural products. The interaction Ni/polyphenols/lipopolysaccharides will also be discussed.

Cite this article as:

Magrone Thea *, Salvatore Rosaria , Spagnoletta Anna , Magrone Manrico , Russo Antonio Matteo and Jirillo Emilio , In Vitro Effects of Nickel on Healthy Non-Allergic Peripheral Blood Mononuclear Cells. The Role of Red Grape Polyphenols, Endocrine, Metabolic & Immune Disorders - Drug Targets 2017; 17 (2) . https://dx.doi.org/10.2174/1871530317666170713145350

DOI https://dx.doi.org/10.2174/1871530317666170713145350	Print ISSN 1871-5303
Publisher Name Bentham Science Publisher	Online ISSN 2212-3873