The Regulatory Functions of a New Tetrapeptide from the Bursa of Fabricius on AIV Vaccine Immunization and Antibody Production

Title:The Regulatory Functions of a New Tetrapeptide from the Bursa of Fabricius on AIV Vaccine Immunization and Antibody Production

Volume: 24 Issue: 7

Author(s): Man Man Zong, Chuan Jie Zhou, Yuan Nan Yu, Yang Zheng, Xiu Li Feng *, Pu Yan Chen and Mei Yang

Affiliation:

• Key Laboratory of Animal Microbiology of China’s Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing 210095,China

Keywords: Bursal tetrapeptide, immunization model, immune response, antibody production, signal pathways, hybridoma.

Abstract: Background: Understanding the regulatory functions of the biological peptide from the humoral central immune organ bursa of Fabricius on vaccine immune responses and antibody production is of vital importance.

Objectives: Here we thoroughly verified the immunomodulatory functions of the new tetrapeptide BP4 from the bursa of Fabricius on vaccine immune responses in mice and chicken immunizaiton model, and on potential intracellular signaling during antibody production.

Method: BP4 was isolated and identified by Reverse Phase High Performance Liquid Chromatography and matrix-assisted laser desorption ionization time of flight mass spectrometry. immunomodulatory functions of BP4 was verified by AIV vaccine immunization on mice and chickens regarding roles in vivo, by monitoring the impact of signalling inhibitors in hybridoma cells on antibody production in vitro.

Results: Our investigation revealed the strong inducing roles of new isolated BP4 on immune responses in mice immunization, the immunomodulatory effects in the immunized chicken, four potential key intracellular signaling during antibody production in hybrdoma cells.

Conclusion: The new bursal-derived peptide BP4 was isolated and identified, and the immunomodulatory effects on antigen-specific immune responses in vivo and in vitro were verified, suggesting BP4 might be highly relevant to the humoral immune responses, and PI3K/Akt, p38 MAPK, NF-κB and tyrosine phosphorylation signaling might be the key activated intracellular signaling during antibody production during BP4 stimulation, which provided a novel potential adjuvant candidate for vaccine immunization improvement and precaution on animal epidemic disease.

Cite this article as:

Zong Man Man , Zhou Jie Chuan, Yu Nan Yuan , Zheng Yang, Feng Li Xiu *, Chen Yan Pu and Yang Mei , The Regulatory Functions of a New Tetrapeptide from the Bursa of Fabricius on AIV Vaccine Immunization and Antibody Production, Protein & Peptide Letters 2017; 24 (7) . https://dx.doi.org/10.2174/0929866524666170426112205

DOI https://dx.doi.org/10.2174/0929866524666170426112205	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305