Abstract
Trypsin, chymotrypsin and elastase were combined to hydrolyze scorpion Buthus marstesii Karsch powders to prepare scorpion peptides (SP). Orthogonal design experiment L16 (44) was used to optimize variables of enzymolysis temperature, time, pH and enzyme concentration. Degree of hydrolysis (DH) and inhibition rate (IR) of A549 cells were selected as analysis indicators. The optimum enzymolysis conditions were determined as follows: enzymolysis temperature 40°C, time 3h, pH 8.5 and enzyme concentration of each 2000U. The anti-tumor activity of SP was determined on a panel of representative cell lines (A549, MCF7 and EC109) using MTT assay. In vitro, SP significantly inhibited the proliferation of A549, which was more obvious than MCF-7 and EC109. Further experiments on cell apoptosis and cell cycle analysis revealed that SP induce apoptosis and arrest cell cycle progression in S phase.
Keywords: Orthogonal design experiment, enzymolysis conditions, anti-tumor activity, cell apoptosis, cell cycle analysis.
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Current Signal Transduction Therapy
Title:Optimization of the Enzymolysis Conditions for Scorpion Peptides and Evaluation of its Antitumor Activity
Volume: 11 Issue: 1
Author(s): Fangwen Jiao, Jihui Wang, Yuhong Liu, Yan Zou, Wei Jia, Panpan Zhang, Jing Sun, Youwei Xu and Junxiang Wang
Affiliation:
Keywords: Orthogonal design experiment, enzymolysis conditions, anti-tumor activity, cell apoptosis, cell cycle analysis.
Abstract: Trypsin, chymotrypsin and elastase were combined to hydrolyze scorpion Buthus marstesii Karsch powders to prepare scorpion peptides (SP). Orthogonal design experiment L16 (44) was used to optimize variables of enzymolysis temperature, time, pH and enzyme concentration. Degree of hydrolysis (DH) and inhibition rate (IR) of A549 cells were selected as analysis indicators. The optimum enzymolysis conditions were determined as follows: enzymolysis temperature 40°C, time 3h, pH 8.5 and enzyme concentration of each 2000U. The anti-tumor activity of SP was determined on a panel of representative cell lines (A549, MCF7 and EC109) using MTT assay. In vitro, SP significantly inhibited the proliferation of A549, which was more obvious than MCF-7 and EC109. Further experiments on cell apoptosis and cell cycle analysis revealed that SP induce apoptosis and arrest cell cycle progression in S phase.
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Jiao Fangwen, Wang Jihui, Liu Yuhong, Zou Yan, Jia Wei, Zhang Panpan, Sun Jing, Xu Youwei and Wang Junxiang, Optimization of the Enzymolysis Conditions for Scorpion Peptides and Evaluation of its Antitumor Activity, Current Signal Transduction Therapy 2016; 11 (1) . https://dx.doi.org/10.2174/1574362411666160517113916
DOI https://dx.doi.org/10.2174/1574362411666160517113916 |
Print ISSN 1574-3624 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-389X |
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