Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application

Title:Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application

Volume: 23 Issue: 3

Author(s): Kentaro Shiraki, Takaaki Kurinomaru and Shunsuke Tomita

Affiliation:

Keywords: Antibody, enzyme, enrichment, polyelectrolyte, enzyme activity, aggregation, precipitation.

Abstract: A polyelectrolyte is a polymer composed of repeating units of an electrolyte group that enables reversible complex formation with proteins in aqueous solutions. This review introduces “wrap-and-strip” technology of protein–polyelectrolyte complex (PPC) by noncovalent interaction. Storage: protein is stabilized against physical and chemical stresses. Enrichment: precipitation through PPC can be used as an enrichment method without irreversible unfolding. Catalytic activity switch: a complementary charged pair of polyelectrolytes functions as a reversible enzyme activity switch. Hyperactivation: a specific combination of a polyelectrolyte and substrate enhances enzyme activity by one order of magnitude compared with an enzyme alone. Stabilization: PPC increases protein stability against chemical and physical stresses, such as covalently modified polyethylene glycosylated protein. Simple PPC-based technology can expand the applicable fields of soluble proteins in aqueous solutions.

Cite this article as:

Shiraki Kentaro, Kurinomaru Takaaki and Tomita Shunsuke, Wrap-and-Strip Technology of Protein–Polyelectrolyte Complex for Biomedical Application, Current Medicinal Chemistry 2016; 23 (3) . https://dx.doi.org/10.2174/0929867323666151127201126